ArticleNucleic acids research2002
Generating segmental mutations in haloalkane dehalogenase: a novel part in the directed evolution toolbox.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 57 citations in OpenAlex.
- Enzyme miniaturization: Revolutionizing future biocatalysts.Biotechnology advances · 2025Review
- Vector redesign and in-droplet cell-growth improves enrichment and recovery in live Escherichia coli.Microbial biotechnology · 2022Article
- Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis.Nature communications · 2020Article
- Conformational changes allow processing of bulky substrates by a haloalkane dehalogenase with a small and buried active site.The Journal of biological chemistry · 2018Article
- Biochemical characterization of two haloalkane dehalogenases: DccA from Caulobacter crescentus and DsaA from Saccharomonospora azurea.Protein science : a publication of the Protein Society · 2016Article
- RAISE: a simple and novel method of generating random insertion and deletion mutations.Nucleic acids research · 2006Article
- Laboratory-directed protein evolution.Microbiology and molecular biology reviews : MMBR · 2005Review
- Two rhizobial strains, Mesorhizobium loti MAFF303099 and Bradyrhizobium japonicum USDA110, encode haloalkane dehalogenases with novel structures and substrate specificities.Applied and environmental microbiology · 2005Article
- Protein evolution by codon-based random deletions.Nucleic acids research · 2004Article
- Chemical and biochemical strategies for the randomization of protein encoding DNA sequences: library construction methods for directed evolution.Nucleic acids research · 2004Review
- Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers.Nucleic acids research · 2003Article
- Diversity of nitrile hydratase and amidase enzyme genes in Rhodococcus erythropolis recovered from geographically distinct habitats.Applied and environmental microbiology · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Directed evolution techniques allow us to genuinely mimic molecular evolution in vitro. To enhance this imitation of natural evolutionary processes on a laboratory scale in even more detail, we developed an in vitro method for the generation of random deletions and repeats. The pairwise fusion of two fragments of the same gene that are truncated by exonuclease BAL-31 either at the 3' or 5' side results in a deletion or a repeat at the fusion point. Although in principle the method randomly covers the whole gene, it can also be limited to a predefined area in the sequence by controlling the level of the initial truncation. To test the procedure and to illustrate its potential, we used haloalkane dehalogenase from Xanthobacter autotrophicus GJ10 (DhlA) as a model enzyme, since the adaptation of this enzyme towards new substrates is known to occur via the generation of this type of mutation. The results show that the mutagenesis method presented here is an effective tool for accessing formerly unexplorable sequence space and can contribute to the success of future directed evolution experiments.
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