ArticleGenome research2002
Functional genomics on potato virus A: virus genome-wide map of sites essential for virus propagation.
Article in Genome research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- Scanning the horizon: deep mutational scanning approaches in virology.Journal of virology · 2026Review
- A conserved lysine/arginine-rich motif is essential for the autophagic degradation of potyviral 6K1 protein and virus infection.Journal of virology · 2025Article
- Review
- Article
- Pest categorisation of potato virus A (non-EU isolates).EFSA journal. European Food Safety Authority · 2020Article
- Infectivity of cloned begomoviral DNAs: an appraisal.Virusdisease · 2019Article
- Mu transpososome activity-profiling yields hyperactive MuA variants for highly efficient genetic and genome engineering.Nucleic acids research · 2018Article
- Tobacco vein banding mosaic virus 6K2 Protein Hijacks NbPsbO1 for Virus Replication.Scientific reports · 2017Article
- Protein composition of 6K2-induced membrane structures formed during Potato virus A infection.Molecular plant pathology · 2016Article
- Plum Pox Virus 6K1 Protein Is Required for Viral Replication and Targets the Viral Replication Complex at the Early Stage of Infection.Journal of virology · 2016Article
- Recombination of strain O segments to HCpro-encoding sequence of strain N of Potato virus Y modulates necrosis induced in tobacco and in potatoes carrying resistance genes Ny or Nc.Molecular plant pathology · 2015Article
- An assay to monitor the activity of DNA transposition complexes yields a general quality control measure for transpositional recombination reactions.Mobile genetic elements · 2014Article
- Article
- Intracellular coordination of potyviral RNA functions in infection.Frontiers in plant science · 2014Review
- High-resolution functional profiling of the norovirus genome.Journal of virology · 2012Article
- Eukaryotic translation initiation factor 4E-mediated recessive resistance to plant viruses and its utility in crop improvement.Molecular plant pathology · 2012Review
- P1 peptidase--a mysterious protein of family Potyviridae.Journal of biosciences · 2011Review
- Article
- Universal platform for quantitative analysis of DNA transposition.Mobile DNA · 2010Article
- Involvement of the plant nucleolus in virus and viroid infections: parallels with animal pathosystems.Advances in virus research · 2010Review
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transposition-based in vitro insertional mutagenesis strategies provide promising new approaches for functional characterization of any cloned gene or genome region. We have extended the methodology and scope of such analysis to a complete viral genome. To map genome regions both essential and nonessential for Potato virus A propagation, we generated a genomic 15-bp insertion mutant library utilizing the efficient in vitro DNA transposition reaction of phage Mu. We then determined the proficiency of 1125 mutants to propagate in tobacco protoplasts by using a genetic footprinting strategy that simultaneously mapped the genomic insertion sites. Over 300 sites critical for virus propagation were identified, and many of them were located in positions previously not assigned to any viral functions. Many genome regions tolerated insertions indicating less important sites for virus propagation and thus pinpointed potential locations for further genome manipulation. The methodology described is applicable to a detailed functional analysis of any viral nucleic acid cloned as DNA and can be used to address many different processes during viral infection cycles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.