ArticleProceedings of the National Academy of Sciences of the United States of America2002
Phosphate backbone neutralization increases duplex DNA flexibility: a model for protein binding.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 54 citations in OpenAlex.
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- Targeting BRPF3 moderately reverses olaparib resistance in high grade serous ovarian carcinoma.Molecular carcinogenesis · 2023Article
- Pre-concentration of microRNAs by LNA-modified magnetic beads for enhancement of electrochemical detection.Scientific reports · 2021Article
- Effect of DNA Flexibility on Complex Formation of a Cationic Nanoparticle with Double-Stranded DNA.ACS omega · 2021Article
- Base-Pairs' Correlated Oscillation Effects on the Charge Transfer in Double-Helix B-DNA Molecules.Materials (Basel, Switzerland) · 2020Article
- Probing the Behaviour of Cas1-Cas2 upon Protospacer Binding in CRISPR-Cas Systems using Molecular Dynamics Simulations.Scientific reports · 2019Article
- How methyl-sugar interactions determine DNA structure and flexibility.Nucleic acids research · 2019Article
- Ligand Binding Induces Agonistic-Like Conformational Adaptations in Helix 12 of Progesterone Receptor Ligand Binding Domain.Frontiers in chemistry · 2019Article
- Facilitated Diffusion Mechanisms in DNA Base Excision Repair and Transcriptional Activation.Chemical reviews · 2018Review
- Differential sensitivity to methylated DNA by ETS-family transcription factors is intrinsically encoded in their DNA-binding domains.Nucleic acids research · 2016Article
- Analysis of heat-labile sites generated by reactions of depleted uranium and ascorbate in plasmid DNA.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2014Article
- DNA curvature and flexibility in vitro and in vivo.Quarterly reviews of biophysics · 2010Review
- Site-specific DNA structural and dynamic features revealed by nucleotide-independent nitroxide probes.Biochemistry · 2009Article
- Examining the ribonuclease H primer grip of HIV-1 reverse transcriptase by charge neutralization of RNA/DNA hybrids.Nucleic acids research · 2008Article
- Rigid spin-labeled nucleoside C: a nonperturbing EPR probe of nucleic acid conformation.Nucleic acids research · 2008Article
- Evolution of acceptor stem tRNA recognition by class II prolyl-tRNA synthetase.Nucleic acids research · 2008Article
- Site-directed spin labeling studies on nucleic acid structure and dynamics.Progress in nucleic acid research and molecular biology · 2008Review
- Effect of lesions on the dynamics of DNA on the picosecond and nanosecond timescales using a polarity sensitive probe.Nucleic acids research · 2004Article
- Origin of the intrinsic rigidity of DNA.Nucleic acids research · 2004Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
An important component of protein-DNA recognition is the charge neutralization of DNA backbone phosphates and subsequent protein-induced DNA bending. Replacement of phosphates by neutral methylphosphonates has previously been shown to be a model for protein-induced bending. In addition to bending, the neutralization process may change the inherent flexibility of the DNA--a feature never before tested. We have developed a method to measure the differential flexibility of duplex DNA when methylphosphonate substitutions are made and find that the local flexibility is increased up to 40%. These results imply that backbone-neutralization-dependent DNA flexibility augments DNA-binding motifs in protein-DNA recognition processes.
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