ArticleThe Journal of general physiology2002
The mechanism of ciliary stimulation by acetylcholine: roles of calcium, PKA, and PKG.
Article in The Journal of general physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 69 citations in OpenAlex.
- Pharmacological regulation of airway ciliary motility: endogenous mediators, pharmaceutical agents, and natural compounds with a focus on ginsenoside Rd.Journal of natural medicines · 2026Review
- Caffeine citrate increases ciliary beat frequency in human respiratory epithelial cells.Molecular and cellular pediatrics · 2026Article
- Respiratory Ciliary Beat Frequency in COPD: Balancing Oxidative Stress and Pharmacological Treatment.Antioxidants (Basel, Switzerland) · 2025Review
- Identification of Nicotinic Acetylcholine Receptor for N-Acetylcysteine to Rescue Nicotine-induced Injury Using Beating Cilia in Primary Tissue Derived Airway Organoids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A 20:1 synergetic mixture of cafedrine/theodrenaline accelerates particle transport velocity in murine tracheal epithelium via IPFrontiers in pharmacology · 2023Article
- Nicotinic Acetylcholine Receptors in the Respiratory Tract.Molecules (Basel, Switzerland) · 2021Review
- First contact: the role of respiratory cilia in host-pathogen interactions in the airways.American journal of physiology. Lung cellular and molecular physiology · 2020Review
- Respiratory Cilia as a Therapeutic Target of Phosphodiesterase Inhibitors.Frontiers in pharmacology · 2020Review
- Construction of a Suite of Computable Biological Network Models Focused on Mucociliary Clearance in the Respiratory Tract.Frontiers in genetics · 2019Article
- Exploring the Role of Fallopian Ciliated Cells in the Pathogenesis of High-Grade Serous Ovarian Cancer.International journal of molecular sciences · 2018Review
- The purplish bifurcate mussel Mytilisepta virgata gene expression atlas reveals a remarkable tissue functional specialization.BMC genomics · 2017Article
- Effects of histamine on ciliary beat frequency of ciliated cells from guinea pigs nasal mucosa.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2015Article
- Cyclic GMP and Cilia Motility.Cells · 2015Review
- Expression and function of a novel variant of estrogen receptor-α36 in murine airways.American journal of respiratory cell and molecular biology · 2011Article
- Computational modelling elucidates the mechanism of ciliary regulation in health and disease.BMC systems biology · 2011Article
- The forces applied by cilia depend linearly on their frequency due to constant geometry of the effective stroke.Biophysical journal · 2008Article
- Effects of cigarette smoke and alcohol on ciliated tracheal epithelium and inflammatory cell recruitment.American journal of respiratory cell and molecular biology · 2007Article
- Substance P and acetylcholine are co-localized in the pathway mediating mucociliary activity in Rana pipiens.Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology · 2007Article
- Intracellular Ca2+ regulates the phosphorylation and the dephosphorylation of ciliary proteins via the NO pathway.The Journal of general physiology · 2004Article
- Regulation of human airway ciliary beat frequency by intracellular pH.The Journal of physiology · 2004Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stimulation of ciliary cells through muscarinic receptors leads to a strong biphasic enhancement of ciliary beat frequency (CBF). The main goal of this work is to delineate the chain of molecular events that lead to the enhancement of CBF induced by acetylcholine (ACh). Here we show that the Ca(2+), cGMP, and cAMP signaling pathways are intimately interconnected in the process of cholinergic ciliary stimulation. ACh induces profound time-dependent increase in cGMP and cAMP concentrations mediated by the calcium-calmodulin complex. The initial strong CBF enhancement in response to ACh is mainly governed by PKG and elevated calcium. The second phase of CBF enhancement induced by ACh, a stable moderately elevated CBF, is mainly regulated by PKA in a Ca(2+)-independent manner. Inhibition of either guanylate cyclase or of PKG partially attenuates the response to ACh of [Ca(2+)](i), but completely abolishes the response of CBF. Inhibition of PKA moderately attenuates and significantly shortens the responses to ACh of both [Ca(2+)](i) and CBF. In addition, PKA facilitates the elevation in [Ca(2+)](i) and cGMP levels induced by ACh, whereas an unimpeded PKG activity is essential for CBF enhancement mediated by either Ca(2+) or PKA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.