ArticleThe Journal of physiology2002
Contractile responses of smooth muscle cells differentiated from rat neural stem cells.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 49 citations in OpenAlex.
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- Towards clinical application of tissue engineering for erectile penile regeneration.Nature reviews. Urology · 2019Review
- Induction of myosin light chain kinase and CPI-17 by TGF-β accelerates contractile activity in intestinal epithelial cells.The Journal of veterinary medical science · 2018Article
- The Biological Role of NestinFrontiers in cell and developmental biology · 2018Review
- Vascular Transdifferentiation in the CNS: A Focus on Neural and Glioblastoma Stem-Like Cells.Stem cells international · 2016Review
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- Human amniotic fluid stem cell differentiation along smooth muscle lineage.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Bioprinting for stem cell research.Trends in biotechnology · 2013Review
- Impaired neural differentiation of induced pluripotent stem cells generated from a mouse model of Sandhoff disease.PloS one · 2013Article
- Evaluation of ES-derived neural progenitors as a potential source for cell replacement therapy in the gut.BMC gastroenterology · 2012Article
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- A new alternative mechanism in glioblastoma vascularization: tubular vasculogenic mimicry.Brain : a journal of neurology · 2010Article
- Urinary bladder smooth muscle engineered from adipose stem cells and a three dimensional synthetic composite.Biomaterials · 2009Article
- Differentiation of bone marrow mesenchymal stem cells into the smooth muscle lineage by blocking ERK/MAPK signaling pathway.Stem cells and development · 2008Article
- Clonogenic multipotent stem cells in human adipose tissue differentiate into functional smooth muscle cells.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Tissue engineering in urology: where are we going?Current urology reports · 2003Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To characterize the functional differentiation of neural stem cells into smooth muscle cells, multipotent stem cells in the central nervous system (CNS) were isolated from rat embryonic day 14 (E14) cortex and cultured by neurosphere formation in serum-free medium in the presence of 10 ng ml(-1) of basic fibroblast growth factor. Differentiation was induced by the addition of 10 % fetal bovine serum to low-density cultures (2.5 x 10(3) cells cm(-2)). Immunological analyses and reverse transcriptase-polymerase chain reaction indicated that the differentiated cells expressed smooth-muscle-specific marker proteins such as SM-1, SM-2, and SMemb myosin heavy chains, SM-22, basic calponin and alpha-smooth-muscle actin, but not the astrocyte marker glial fibrillary acidic protein. To examine whether smooth-muscle-like cells that are differentiated from CNS stem cells possess the characteristics of contractile smooth muscle, we prepared reconstituted collagen gel fibres and measured their contractile tension. The reconstituted fibres were prepared by thermal gelation of collagen and the differentiated cells. The fibres contracted in response to treatment with KCl (80 mM), ACh (100 microM), endothelin-1 (10 nM), endothelin-2 (10 nM), and prostaglandin F2alpha (100 microM). ACh-induced contraction was partially inhibited by the L-type voltage-dependent Ca(2+) channel inhibitor nifedipine and by the intracellular Ca(2+) chelator 1,2-bis (2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester, the myosin light chain kinase inhibitor ML-9, the Rho kinase inhibitor Y-27632, dibutyryl cAMP and 8-bromo-cGMP. These results suggest that CNS stem cells give rise to smooth muscle cells in vitro that have an identical contractile function to smooth muscle in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.