ArticleThe Journal of experimental medicine2002
A natural killer T (NKT) cell developmental pathway iInvolving a thymus-dependent NK1.1(-)CD4(+) CD1d-dependent precursor stage.
Article in The Journal of experimental medicine, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 174 papers.
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Who cites it
174 citing papers in PubMed, 363 citations in OpenAlex.
- Beyond subsets: single cell transcriptomics reveals multidimensional regulation of iNKT cells cross tissues and species.Frontiers in immunology · 2026Review
- Single-cell transcriptomic profiling reveals diversity in human iNKT cells across hematologic tissues.Cell reports · 2025Article
- Can invariant Natural Killer T cells drive B cell fate? a look at the humoral response.Frontiers in immunology · 2025Review
- Single-cell transcriptomics reveal different maturation stages and sublineage commitment of human thymic invariant natural killer T cells.Journal of leukocyte biology · 2024Article
- Insights into the heterogeneity of iNKT cells: tissue-resident and circulating subsets shaped by local microenvironmental cues.Frontiers in immunology · 2024Review
- Thymic development of human natural killer T cells: recent advances and implications for immunotherapy.Frontiers in immunology · 2024Review
- Eed-dependent histone modification orchestrates the iNKT cell developmental program alleviating liver injury.Frontiers in immunology · 2024Article
- Markers and makers of NKT17 cells.Experimental & molecular medicine · 2023Review
- Impaired thymic iNKT cell differentiation at early precursor stage in murine haploidentical bone marrow transplantation with GvHD.Frontiers in immunology · 2023Article
- Thymus-derived hormonal and cellular control of cancer.Frontiers in endocrinology · 2023Review
- METTL14-dependent mCell reports · 2022Article
- Effects of TNF-α deletion on iNKT cell development, activation, and maturation in the steady-state and chronic alcohol-consuming mice.Journal of leukocyte biology · 2022Article
- Adipocyte CD1d Gene Transfer Induces T Cell Expansion and Adipocyte Inflammation in CD1d Knockout Mice.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- The impact of the gut microbiota on T cell ontogeny in the thymus.Cellular and molecular life sciences : CMLS · 2022Review
- Current insights in mouse iNKT and MAIT cell development using single cell transcriptomics data.Seminars in immunology · 2022Review
- In vivo availability of the cytokine IL-7 constrains the survival and homeostasis of peripheral iNKT cells.Cell reports · 2022Article
- Development of αβ T Cells with Innate Functions.Advances in experimental medicine and biology · 2022Article
- Protein abundance of the cytokine receptor γc controls the thymic generation of innate-like T cells.Cellular and molecular life sciences : CMLS · 2021Article
- Modulation of TCR signalling components occurs prior to positive selection and lineage commitment in iNKT cells.Scientific reports · 2021Article
- The impact of stress on the transcriptomic signature of iNKT1 cells.Biochemistry and biophysics reports · 2021Article
114 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of CD1d-dependent natural killer T (NKT) cells is poorly understood. We have used both CD1d/alpha-galactosylceramide (CD1d/alphaGC) tetramers and anti-NK1.1 to investigate NKT cell development in vitro and in vivo. Confirming the thymus-dependence of these cells, we show that CD1d/alphaGC tetramer-binding NKT cells, including NK1.1(+) and NK1.1(-) subsets, develop in fetal thymus organ culture (FTOC) and are completely absent in nude mice. Ontogenically, CD1d/alphaGC tetramer-binding NKT cells first appear in the thymus, at day 5 after birth, as CD4(+)CD8(-)NK1.1(-)cells. NK1.1(+) NKT cells, including CD4(+) and CD4(-)CD8(-) subsets, appeared at days 7-8 but remained a minor subset until at least 3 wk of age. Using intrathymic transfer experiments, CD4(+)NK1.1(-) NKT cells gave rise to NK1.1(+) NKT cells (including CD4(+) and CD4(-) subsets), but not vice-versa. This maturation step was not required for NKT cells to migrate to other tissues, as NK1.1(-) NKT cells were detected in liver and spleen as early as day 8 after birth, and the majority of NKT cells among recent thymic emigrants (RTE) were NK1.1(-). Further elucidation of this NKT cell developmental pathway should prove to be invaluable for studying the mechanisms that regulate the development of these cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.