ArticleRNA (New York, N.Y.)2002
The 5' and 3' extremities of the satellite tobacco necrosis virus translational enhancer domain contribute differentially to stimulation of translation.
Article in RNA (New York, N.Y.), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Translation of Plant RNA Viruses.Viruses · 2021Review
- The 3' Untranslated Region of a Plant Viral RNA Directs Efficient Cap-Independent Translation in Plant and Mammalian Systems.Pathogens (Basel, Switzerland) · 2019Article
- Structural and Functional Diversity of Plant Virus 3'-Cap-Independent Translation Enhancers (3'-CITEs).Frontiers in plant science · 2017Review
- Non-canonical Translation in Plant RNA Viruses.Frontiers in plant science · 2017Review
- Article
- Linking Α to Ω: diverse and dynamic RNA-based mechanisms to regulate gene expression by 5'-to-3' communication.F1000Research · 2016Review
- Cis- and trans-regulation of luteovirus gene expression by the 3' end of the viral genome.Virus research · 2015Review
- Revealing the density of encoded functions in a viral RNA.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- 3' cap-independent translation enhancers of plant viruses.Annual review of microbiology · 2013Review
- Untranslated regions of diverse plant viral RNAs vary greatly in translation enhancement efficiency.BMC biotechnology · 2012Article
- Tombusvirus recruitment of host translational machinery via the 3' UTR.RNA (New York, N.Y.) · 2010Article
- Satellite RNAs and Satellite Viruses of Plants.Viruses · 2009Article
- Advances in RNA structure prediction from sequence: new tools for generating hypotheses about viral RNA structure-function relationships.Journal of virology · 2009Review
- Structure of a viral cap-independent translation element that functions via high affinity binding to the eIF4E subunit of eIF4F.The Journal of biological chemistry · 2009Article
- The 3' cap-independent translation element of Barley yellow dwarf virus binds eIF4F via the eIF4G subunit to initiate translation.RNA (New York, N.Y.) · 2008Article
- The amazing diversity of cap-independent translation elements in the 3'-untranslated regions of plant viral RNAs.Biochemical Society transactions · 2007Review
- Cap-independent translation of plant viral RNAs.Virus research · 2006Review
- The 3' untranslated region of tobacco necrosis virus RNA contains a barley yellow dwarf virus-like cap-independent translation element.Journal of virology · 2004Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The translational enhancer domain (TED) of satellite tobacco necrosis virus (STNV) RNA stimulates translation of uncapped RNAs autonomously. Here we set out to identify the 5' and 3' extremities of TED and features of these sequences with respect to translation. We found that both in wheat germ extract and in tobacco protoplasts, the 5' border is confined to 3 nt. Mutational analysis revealed that the autonomous function of TED is sensitive to 5' flanking sequences. At the 3' end of TED, 23 nt have a cumulative, quantitative effect on translation in wheat germ extract, whereas in tobacco protoplasts, the most 3' 14 nt of these 23 nt do not enhance translation. The 5' and 3' sequence requirements triggered the development of a new secondary structure model. In this model, TED folds into a phylogenetically conserved stem-loop structure in which the essential 5' nucleotides base-pair with the 3' nucleotides that stimulate translation both in vitro and in vivo. Importantly, the 14 3' nucleotides in TED that stimulate translation in the wheat germ extract only do not require the predicted base-pairing in order to function. The discrepancy between in vitro and in vivo sequence requirements thus correlates with potential base-pairing requirements, opening the possibility that TED contains two functional domains.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.