ArticleNature biotechnology2002
Identification of genes that function in the TNF-alpha-mediated apoptotic pathway using randomized hybrid ribozyme libraries.
Article in Nature biotechnology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed.
- Decoding the Immune Microenvironment of Clear Cell Renal Cell Carcinoma by Single-Cell Profiling to Aid Immunotherapy.Frontiers in immunology · 2022Article
- Immunosuppressive effects of hydroxychloroquine and artemisinin combination therapy via the nuclear factor-κB signaling pathway in lupus nephritis mice.Experimental and therapeutic medicine · 2018Article
- TNF-α-induced Inflammation Stimulates Apolipoprotein-A4 via Activation of TNFR2 and NF-κB Signaling in Kidney Tubular Cells.Scientific reports · 2017Article
- Gene-Set Reduction for Analysis of Major and Minor Gleason Scores Based on Differential Gene-Set Expressions and Biological Pathways in Prostate Cancer.Cancer informatics · 2017Article
- Ethanol consumption as inductor of pancreatitis.World journal of gastrointestinal pharmacology and therapeutics · 2010Article
- Complete, gene-specific siRNA libraries: production and expression in mammalian cells.RNA (New York, N.Y.) · 2005Article
- Catalytic nucleic acid enzymes for the study and development of therapies in the central nervous system: Review Article.Gene therapy & molecular biology · 2005Article
- Identification of cellular cofactors for human immunodeficiency virus replication via a ribozyme-based genomics approach.Journal of virology · 2004Article
- An RNA-dependent protein kinase is involved in tunicamycin-induced apoptosis and Alzheimer's disease.The EMBO journal · 2004Article
- Cell migration and metastasis as targets of small RNA-based molecular genetic analyses.Journal of muscle research and cell motility · 2004Review
- Effects on RNAi of the tight structure, sequence and position of the targeted region.Nucleic acids research · 2004Article
- Toward a functional annotation of the human genome using artificial transcription factors.Genome research · 2003Article
- Expressing functional siRNAs in mammalian cells using convergent transcription.BMC biotechnology · 2003Article
- Identification of genes involved in cell invasion by using a library of randomized hybrid ribozymes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Short hairpin type of dsRNAs that are controlled by tRNA(Val) promoter significantly induce RNAi-mediated gene silencing in the cytoplasm of human cells.Nucleic acids research · 2003Article
- Confirmation by FRET in individual living cells of the absence of significant amyloid beta -mediated caspase 8 activation.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- A functional gene discovery in the Fas-mediated pathway to apoptosis by analysis of transiently expressed randomized hybrid-ribozyme libraries.Nucleic acids research · 2002Article
Corrections and comments
- Retraction · 2006-09-01Breach of Policy by Author · Concerns/Issues about Data · Ethical Violations by Author ·
- Retracted
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Now that the sequences of many genomes are available, methods are required for the rapid identification of functional genes. We describe here a simple system for the isolation of genes that function in the tumor necrosis factor-alpha (TNF-alpha)-mediated pathway of apoptosis, using RNA helicase-associated ribozyme libraries with randomized substrate-binding arms. Because target-site accessibility considerably limits the effective use of intracellular ribozymes, the effectiveness of a conventional ribozyme library has been low. To overcome this obstacle, we attached to ribozymes an RNA motif (poly(A)-tail) able to interact with endogenous RNA helicase(s) so that the resulting helicase-attached, hybrid ribozymes can more easily attack target sites regardless of their secondary or tertiary structures. When the phenotype of cells changes upon introduction of a ribozyme library, genes responsible for these changes may be identified by sequencing the active ribozyme clones. In the case of TNF-alpha-mediated apoptosis, when a ribozyme library was introduced into MCF-7 cells, surviving clones were completely or partially resistant to TNF-alpha-induced apoptosis. We identified many pro-apoptotic genes and partial sequences of previously uncharacterized genes using this method. Our gene discovery system should be generally applicable to the identification of functional genes in various systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.