ArticleJournal of clinical microbiology2002
Usefulness of multilocus sequence typing for characterization of clinical isolates of Candida albicans.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed, 199 citations in OpenAlex.
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- Multilocus sequence typing of Candida albicans oral isolates reveals high genetic relatedness of mother-child dyads in early life.PloS one · 2024Article
- Molecular characterization of fungal endophthalmitis and keratitis caused by yeasts.Medical mycology · 2022Article
- Genotyping ofCurrent medical mycology · 2022Article
- Specific associations between fungi and bacteria in broncho-alveolar aspirates from mechanically ventilated intensive care unit patients.Virulence · 2022Article
- Staphylococcus aureus genotype variation among and within periprosthetic joint infections.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2022Article
- Multilocus Sequence Typing and Antifungal Susceptibility ofFrontiers in veterinary science · 2022Article
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- Multilocus Sequence Typing Reveals Extensive Genetic Diversity of the Emerging Fungal PathogenFrontiers in cellular and infection microbiology · 2021Article
- Molecular Epidemiology, Genetic Diversity, and Antifungal Susceptibility of Major Pathogenic Dermatophytes Isolated From Human Dermatophytosis.Frontiers in microbiology · 2021Article
- Diversity of the diploid sequence type ofNew microbes and new infections · 2020Article
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- Investigation of Genetic Relationships BetweenFrontiers in microbiology · 2019Article
- A Predominance of Clade 17Frontiers in microbiology · 2019Article
- Multilocus sequence typing of Candida albicans isolates from the oral cavities of patients undergoing haemodialysis.Scientific reports · 2018Article
- Gene flow contributes to diversification of the major fungal pathogen Candida albicans.Nature communications · 2018Article
29 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular characterization of Candida albicans isolates is essential for understanding the epidemiology of nosocomial infections caused by this yeast. Here, we investigated the potential value of multilocus sequence typing (MLST) for characterizing epidemiologically related or unrelated C. albicans strains of various clinical origins. Accordingly, we sequenced the internal regions (loci) of six selected housekeeping genes of 40 C. albicans clinical isolates and 2 reference strains. In all, 68 polymorphic nucleotide sites were identified, of which 65 were found to be heterozygous in at least one isolate. Ten to 24 different genotypes were observed at the different loci, resulting, when combined, in 39 unique genotype combinations or diploid sequence types (DSTs). When MLST was applied to 26 epidemiologically unrelated isolates and the 2 reference strains, it allowed the identification of 27 independent DSTs, thus demonstrating a discriminatory power of 99.7. Using multidimensional scaling together with the minimum spanning tree method to analyze interstrain relationships, we identified six groups of genetically related isolates on the basis of bootstrap values of greater than 900. Application of MLST to 14 epidemiologically related isolates showed that those recovered from patients in the same hospital ward during the same 3 months had specific DSTs, although 73% of these isolates were genetically very close. This suggests that MLST can trace minute variations in the sequences of related isolates. Overall, MLST proved to be a highly discriminatory and stable method for unambiguous characterization of C. albicans.
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