ArticleJournal of clinical microbiology2002
Comparison of DNA hybridization and PCR assays for detection of putative pathogenic enteroadherent Escherichia coli.
Article in Journal of clinical microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- PCR diagnostics are insufficient for the detection of Diarrhoeagenic Escherichia coli in Ibadan, Nigeria.PLOS global public health · 2023Article
- Comparative analysis of virulence determinants, phylogroups, and antibiotic susceptibility patterns of typical versus atypical Enteroaggregative E. coli in India.PLoS neglected tropical diseases · 2020Article
- Analysis of the Virulence Profile and Phenotypic Features of Typical and Atypical EnteroaggregativeFrontiers in cellular and infection microbiology · 2020Article
- Genetic analysis of enteropathogenic Escherichia coli (EPEC) adherence factor (EAF) plasmid reveals a new deletion within the EAF probe sequence among O119 typical EPEC strains.BMC microbiology · 2015Article
- Development of a polymerase chain reaction assay for the rapid detection of the oral pathogenic bacterium, Selenomonas noxia.BMC oral health · 2015Article
- Adherence and virulence genes of Escherichia coli from children diarrhoea in the Brazilian Amazon.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2015Article
- Rapid genetic typing of diarrheagenic Escherichia coli using a two-tube modified molecular beacon based multiplex real-time PCR assay and its clinical application.Annals of clinical microbiology and antimicrobials · 2014Article
- Detection and genetic analysis of the enteroaggregative Escherichia coli heat-stable enterotoxin (EAST1) gene in clinical isolates of enteropathogenic Escherichia coli (EPEC) strains.BMC microbiology · 2014Article
- Genotypic and phenotypic analysis of diarrheagenic Escherichia coli strains isolated from Brazilian children living in low socioeconomic level communities.BMC infectious diseases · 2013Article
- Evidence of pathogenic subgroups among atypical enteropathogenic Escherichia coli strains.Journal of clinical microbiology · 2009Article
- Phylogenetic backgrounds and virulence profiles of atypical enteropathogenic Escherichia coli strains from a case-control study using multilocus sequence typing and DNA microarray analysis.Journal of clinical microbiology · 2008Article
- Diarrheagenic Escherichia coli and Shigella strains isolated from children in a hospital case-control study in Hanoi, Vietnam.Journal of clinical microbiology · 2008Article
- Comparison between O serotyping method and multiplex real-time PCR to identify diarrheagenic Escherichia coli in Taiwan.Journal of clinical microbiology · 2007Article
- Typical enteroaggregative and atypical enteropathogenic types of Escherichia coli are the most prevalent diarrhea-associated pathotypes among Brazilian children.Journal of clinical microbiology · 2007Article
- Identification of diarrheagenic Escherichia coli isolated from infants and children in Dar es Salaam, Tanzania.BMC infectious diseases · 2007Article
- Identification of virulence genes linked with diarrhea due to atypical enteropathogenic Escherichia coli by DNA microarray analysis and PCR.Journal of clinical microbiology · 2006Article
- Heterogeneity among strains of diffusely adherent Escherichia coli isolated in Brazil.Journal of clinical microbiology · 2005Article
- Evaluation of multiplex PCRs for diagnosis of infection with diarrheagenic Escherichia coli and Shigella spp.Journal of clinical microbiology · 2004Article
- Enteroaggregative Escherichia coli virulence factors are found to be associated with infantile diarrhea in Brazil.Journal of clinical microbiology · 2004Article
- Molecular epidemiology of the iron utilization genes of enteroaggregative Escherichia coli.Journal of clinical microbiology · 2004Article
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5 authors.
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Abstract
The correlation of the different adherence patterns with DNA probes and PCR primers for the identification of Escherichia coli was analyzed in isolates from children, less than 2 years of age with or without diarrhea, from different regions of Brazil. A total of 1,428 isolates obtained from 338 patients and 322 control children were studied. The enteropathogenic E. coli (EPEC) adherence factor (EAF) probe was shown to be as good as the HEp-2 adhesion assay for the detection of typical EPEC strains. The DNA probes used to detect diffusely adhering E. coli and enteroaggregative E. coli (EAEC) showed low sensitivities (64 and 50%, respectively), and the best method of identifying these organisms in clinical research remains the HEp-2 adherence assay. The "bundle-forming pilus" (BFP) and the EAEC PCR assays could be used instead of the DNA probes as a screening method for typical EPEC and EAEC carrying the EAEC probe sequence in the clinical laboratory. In our study, only typical EPEC strains that carried EAF and BFP were associated with acute diarrhea.
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