ArticleNucleic acids research2002
Glass bead purification of plasmid template DNA for high throughput sequencing of mammalian genomes.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 39 citations in OpenAlex.
- Use of a Novel Whole Blood Separation and Transport Device for Targeted and Untargeted Proteomics.Biomedicines · 2024Article
- Development of a high-resolution melt-based assay to rapidly detect the azole-resistantCurrent medical mycology · 2023Article
- Homebrew: An economical and sensitive glassmilk-based nucleic-acid extraction method for SARS-CoV-2 diagnostics.Cell reports methods · 2022Article
- Enrichment of low abundance DNA/RNA by oligonucleotide-clicked iron oxide nanoparticles.Scientific reports · 2021Article
- Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA.BioMed research international · 2019Article
- Rapid Regeneration and Reuse of Silica Columns from PCR Purification and Gel Extraction Kits.Scientific reports · 2018Article
- Rapid, Effective DNA Isolation from Osmanthus via Modified Alkaline Lysis.Journal of biomolecular techniques : JBT · 2016Article
- High-throughput, automated extraction of DNA and RNA from clinical samples using TruTip technology on common liquid handling robots.Journal of visualized experiments : JoVE · 2013Article
- Analysis of expressed sequence tags and identification of genes encoding cell-wall-degrading enzymes from the fungivorous nematode Aphelenchus avenae.BMC genomics · 2009Article
- A method for preparing DNA sequencing templates using a DNA-binding microplate.Journal of biomolecular techniques : JBT · 2009Article
- DNA, RNA, and protein extraction: the past and the present.Journal of biomedicine & biotechnology · 2009Review
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
Abstract
To meet the new challenge of generating the draft sequences of mammalian genomes, we describe the development of a novel high throughput 96-well method for the purification of plasmid DNA template using size-fractionated, acid-washed glass beads. Unlike most previously described approaches, the current method has been designed and optimized to facilitate the direct binding of alcohol-precipitated plasmid DNA to glass beads from alkaline lysed bacterial cells containing the insoluble cellular aggregate material. Eliminating the tedious step of separating the cleared lysate significantly simplifies the method and improves throughput and reliability. During a 4 month period of 96-capillary DNA sequencing of the Rattus norvegicus genome at the Baylor College of Medicine Human Genome Sequencing Center, the average success rate and read length derived from >1 800 000 plasmid DNA templates prepared by the direct lysis/glass bead method were 82.2% and 516 bases, respectively. The cost of this direct lysis/glass bead method in September 2001 was approximately 10 cents per clone, which is a significant cost saving in high throughput genomic sequencing efforts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.