ArticleNucleic acids research2002
Multiplex Pyrosequencing.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Evaluation of the correctable decoding sequencing as a new powerful strategy for DNA sequencing.Life science alliance · 2022Article
- Mutant Huntingtin Affects Diabetes and Alzheimer's Markers in Human and Cell Models of Huntington's Disease.Cells · 2019Article
- DNA methylation-based forensic age prediction using artificial neural networks and next generation sequencing.Forensic science international. Genetics · 2017Article
- Multiplex pyrosequencing method to determine CYP2C9*3, VKORC1*2, and CYP4F2*3 polymorphisms simultaneously: its application to a Korean population and comparisons with other ethnic groups.Molecular biology reports · 2014Article
- Isolating single stranded DNA using a microfluidic dialysis device.The Analyst · 2014Article
- Methods for the preparation of large quantities of complex single-stranded oligonucleotide libraries.PloS one · 2014Article
- Effect of polybrominated diphenyl ether (PBDE) treatment on the composition and function of the bacterial community in the sponge Haliclona cymaeformis.Frontiers in microbiology · 2014Article
- Genome-Wide Association Study Reveals Host Genetic Factors for Liver Diseases.Journal of clinical and translational hepatology · 2013Review
- Multiplex allele-specific amplification from whole blood for detecting multiple polymorphisms simultaneously.Genetic testing and molecular biomarkers · 2013Article
- MultiPSQ: a software solution for the analysis of diagnostic n-plexed pyrosequencing reactions.PloS one · 2013Article
- Rapid detection and quantitation of BRAF mutations in hairy cell leukemia using a sensitive pyrosequencing assay.American journal of clinical pathology · 2012Article
- Molecular markers for detection and differentiation of Plasmodium falciparum and Plasmodium vivax in human blood samples by pyrosequencing.Journal of clinical microbiology · 2012Article
- A simple method using PyrosequencingTM to identify de novo SNPs in pooled DNA samples.Nucleic acids research · 2011Article
- Molecular analysis of linezolid resistance in clinical Enterococcus faecium isolates by polymerase chain reaction and pyrosequencing.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011Article
- Emerging molecular assays for detection and characterization of respiratory viruses.Clinics in laboratory medicine · 2009Review
- Weak or no association of TCF7L2 variants with Type 2 diabetes risk in an Arab population.BMC medical genetics · 2008Article
- Analysis of read length limiting factors in Pyrosequencing chemistry.Analytical biochemistry · 2007Article
- Methodological improvements of pyrosequencing technology.Journal of biotechnology · 2006Article
- Direct amplification of single-stranded DNA for pyrosequencing using linear-after-the-exponential (LATE)-PCR.Analytical biochemistry · 2006Article
- Multiplex SNP typing by bioluminometric assay coupled with terminator incorporation (BATI).Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We describe here the development of a new and simple single-tube multiplex Pyrosequencing assay. Genomic DNA or cDNA was employed to PCR amplify region(s) using biotinylated and normal primer(s). Subsequent to capture of PCR products on streptavidin-coated beads, single-stranded DNA separation and hybridization of multiple sequencing primers, Pyrosequencing was performed. The obtained pyrogram resulted in a unique pattern in which the intensity of the signal determined the number of incorporated nucleotide(s). Here, we demonstrate the use of this multiplex Pyrosequencing for single nucleotide polymorphisms genotyping and microbial typing.
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Registered trials
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