ArticleNucleic acids research2002
An assessment of Motorola CodeLink microarray performance for gene expression profiling applications.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
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- Microreactor array device.Scientific reports · 2015Article
- BIOPHYSICAL PROPERTIES OF NUCLEIC ACIDS AT SURFACES RELEVANT TO MICROARRAY PERFORMANCE.Biomaterials science · 2014Article
- Analysis of differentially expressed genes in colorectal adenocarcinoma with versus without metastasis by three-dimensional oligonucleotide microarray.International journal of clinical and experimental pathology · 2014Article
- [Testosterone reduces the expression of keratinization-promoting genes in murine Meibomian glands].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2013Article
- Assessing the detection capacity of microarrays as bio/nanosensing platforms.BioMed research international · 2013Article
- Genome-wide transcriptional response of the archaeon Thermococcus gammatolerans to cadmium.PloS one · 2012Article
- Classification of diet-modulated gene signatures at the colon cancer initiation and progression stages.Digestive diseases and sciences · 2011Article
- Daptomycin resistance mechanisms in clinically derived Staphylococcus aureus strains assessed by a combined transcriptomics and proteomics approach.The Journal of antimicrobial chemotherapy · 2011Article
- Pdx1 and Ngn3 overexpression enhances pancreatic differentiation of mouse ES cell-derived endoderm population.PloS one · 2011Article
- Identification of transcriptional networks responding to pyrroloquinoline quinone dietary supplementation and their influence on thioredoxin expression, and the JAK/STAT and MAPK pathways.The Biochemical journal · 2010Article
- Adipocyte accumulation of long-chain fatty acids in obesity is multifactorial, resulting from increased fatty acid uptake and decreased activity of genes involved in fat utilization.Obesity surgery · 2010Article
- Article
- Evaluation of Candidate Reference Genes for Real-Time Quantitative PCR of Plant Samples Using Purified cDNA as Template.Plant molecular biology reporter · 2009Article
- Time-resolved Förster-resonance-energy-transfer DNA assay on an active CMOS microarray.Biosensors & bioelectronics · 2008Article
- Role of oxygen availability in CFTR expression and function.American journal of respiratory cell and molecular biology · 2008Article
- Advanced genomic data mining.PLoS computational biology · 2008Article
- The effects of estrogen and testosterone on gene expression in the rat mesenteric arteries.Vascular pharmacology · 2007Article
- Genome wide expression analysis in HPV16 cervical cancer: identification of altered metabolic pathways.Infectious agents and cancer · 2007Article
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15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA microarrays enable users to obtain information on differences in transcript abundance on a massively parallel scale. Recently, however, data analyses have revealed potential pitfalls related to image acquisition, variability and misclassifications in replicate measurements, cross-hybridization and sensitivity limitations. We have generated a series of analytical tools to address the manufacturing, detection and data analysis components of a microarray experiment. Together, we have used these tools to optimize performance in an expression profiling study. We demonstrate three significant advantages of the Motorola CodeLink platform: sensitivity of one copy per cell, coefficients of variation of 10% in the hybridization signals across slides and across target preparations, and specificity in distinguishing highly homologous sequences. Slides where oligonucleotide probes are spotted in 6-fold redundancy were used to demonstrate the effect of replication on data quality. Lastly, the differential expression ratios obtained with the CodeLink expression platform were validated against those obtained with quantitative reverse transcription-PCR assays for 54 genes.
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