ArticleNucleic acids research2002
An in vivo selection system for homing endonuclease activity.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Positive Selection Screens for Programmable Endonuclease Activity Using I-SceI.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Engineering altered protein-DNA recognition specificity.Nucleic acids research · 2018Review
- Article
- Homing endonucleases: from genetic anomalies to programmable genomic clippers.Methods in molecular biology (Clifton, N.J.) · 2014Review
- Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleases.Nucleic acids research · 2012Article
- Homing endonucleases: from microbial genetic invaders to reagents for targeted DNA modification.Structure (London, England : 1993) · 2011Review
- Microhomology-mediated and nonhomologous repair of a double-strand break in the chloroplast genome of Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Homing endonucleases: from basics to therapeutic applications.Cellular and molecular life sciences : CMLS · 2010Review
- Directed evolution of homing endonuclease I-SceI with altered sequence specificity.Protein engineering, design & selection : PEDS · 2009Article
- Thermodynamics of DNA target site recognition by homing endonucleases.Nucleic acids research · 2007Article
- Engineering a rare-cutting restriction enzyme: genetic screening and selection of NotI variants.Nucleic acids research · 2006Article
- In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination.Nucleic acids research · 2005Article
- A highly sensitive selection method for directed evolution of homing endonucleases.Nucleic acids research · 2005Article
- In vitro selection of restriction endonucleases by in vitro compartmentalization.Nucleic acids research · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Homing endonucleases are enzymes that catalyze the highly sequence-specific cleavage of DNA. We have developed an in vivo selection in Escherichia coli that links cell survival with homing endonuclease-mediated DNA cleavage activity and sequence specificity. Using this selection, wild-type and mutant variants of three homing endonucleases were characterized without requiring protein purification and in vitro analysis. This selection system may facilitate the study of sequence-specific DNA cleaving enzymes, and selections based on this work may enable the evolution of homing endonucleases with novel activities or specificities.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.