ArticleNucleic acids research2002
Methylome profiling of cancer cells by amplification of inter-methylated sites (AIMS).
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 115 citations in OpenAlex.
- DNA methylation methods: Global DNA methylation and methylomic analyses.Methods (San Diego, Calif.) · 2021Review
- Approaches for the study of epigenetic modifications in the inner ear and related tissues.Hearing research · 2019Review
- Overlapping DNA methylation dynamics in mouse intestinal cell differentiation and early stages of malignant progression.PloS one · 2015Article
- Cancer genomics identifies disrupted epigenetic genes.Human genetics · 2014Review
- Methylation and worker reproduction in the bumble-bee (Bombus terrestris).Proceedings. Biological sciences · 2014Article
- DNA methylation and cancer diagnosis.International journal of molecular sciences · 2013Review
- Deciphering the epigenetic code: an overview of DNA methylation analysis methods.Antioxidants & redox signaling · 2013Review
- Strategies for discovery and validation of methylated and hydroxymethylated DNA biomarkers.Cancer medicine · 2012Review
- Genetic, environmental and stochastic factors in monozygotic twin discordance with a focus on epigenetic differences.BMC medicine · 2012Review
- Dissecting DNA hypermethylation in cancer.FEBS letters · 2011Review
- DNA methylation: a timeline of methods and applications.Frontiers in genetics · 2011Article
- Biomarkers in Barrett's esophagus and esophageal adenocarcinoma: predictors of progression and prognosis.World journal of gastroenterology · 2010Review
- Methods for genome-wide analysis of DNA methylation in intestinal tumors.Mutation research · 2010Review
- DNA fingerprinting techniques for the analysis of genetic and epigenetic alterations in colorectal cancer.Mutation research · 2010Review
- Epigenomics and ovarian carcinoma.Biomarkers in medicine · 2010Review
- Experimental approaches to the study of epigenomic dysregulation in ageing.Experimental gerontology · 2010Review
- Principles and challenges of genomewide DNA methylation analysis.Nature reviews. Genetics · 2010Review
- GAREM, a novel adaptor protein for growth factor receptor-bound protein 2, contributes to cellular transformation through the activation of extracellular signal-regulated kinase signaling.The Journal of biological chemistry · 2009Article
- Epigenomics and breast cancer.Pharmacogenomics · 2008Review
- Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Alterations of the DNA methylation pattern have been related to generalized chromosomal disruption and inactivation of multiple tumor suppressor genes in neoplasia. To screen for tumor-specific alterations and to make a global assessment of methylation status in cancer cells, we have modified the methylated CpG island amplification method to generate easily readable fingerprints representing the cell's DNA methylation profile. The method is based on the differential cleavage of isoschizomers with distinct methylation sensitivity. Specific adaptors are ligated to the methylated ends of the digested genomic DNA. The ligated sequences are amplified by PCR using adaptor- specific primers extended at the 3' end with two to four arbitrarily chosen nucleotidic residues to reduce the complexity of the product. Fingerprints consist of multiple anonymous bands, representing DNA sequences flanked by two methylated sites, which can be isolated and individually characterized. Hybridization of the whole product to metaphase chromosomes revealed that most bands originate from the isochore H3, which identifies the regions of the genome with the highest content of CpG islands and genes. Comparison of the fingerprints obtained from normal colon mucosa, colorectal carcinomas and cell lines revealed tumor-specific alterations that are putative recurrent markers of the disease and include tumor-specific hypo- and hypermethylations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.