ArticleNucleic acids research2002
SmpB functions in various steps of trans-translation.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 85 citations in OpenAlex.
- Review
- Structures of tmRNA and SmpB as they transit through the ribosome.Nature communications · 2021Article
- Trans-translation is essential in the human pathogen Legionella pneumophila.Scientific reports · 2016Article
- Rejection of tmRNA·SmpB after GTP hydrolysis by EF-Tu on ribosomes stalled on intact mRNA.RNA (New York, N.Y.) · 2014Article
- In vivo tmRNA protection by SmpB and pre-ribosome binding conformation in solution.RNA (New York, N.Y.) · 2014Article
- tmRNA-mediated trans-translation as the major ribosome rescue system in a bacterial cell.Frontiers in genetics · 2014Review
- The SmpB C-terminal tail helps tmRNA to recognize and enter stalled ribosomes.Frontiers in microbiology · 2014Review
- Biochemical aspects of bacterial strategies for handling the incomplete translation processes.Frontiers in microbiology · 2014Review
- Mechanism of trans-translation revealed by in vitro studies.Frontiers in microbiology · 2014Review
- Studies on the mechanism of telavancin decreased susceptibility in a laboratory-derived mutant.Microbial drug resistance (Larchmont, N.Y.) · 2013Article
- Article
- The tmRNA ribosome-rescue system.Advances in protein chemistry and structural biology · 2012Review
- Bifunctional transfer-messenger RNA.Biochimie · 2011Review
- tRNA/mRNA Mimicry by tmRNA and SmpB in Trans-Translation.Journal of nucleic acids · 2011Article
- Role of the C-terminal tail of SmpB in the early stage of trans-translation.RNA (New York, N.Y.) · 2010Article
- Accommodation of tmRNA-SmpB into stalled ribosomes: a cryo-EM study.RNA (New York, N.Y.) · 2010Article
- Structural features of the tmRNA-ribosome interaction.RNA (New York, N.Y.) · 2009Article
- The SmpB-tmRNA tagging system plays important roles in Streptomyces coelicolor growth and development.PloS one · 2009Article
- The highest affinity binding site of small protein B on transfer messenger RNA is outside the tRNA domain.RNA (New York, N.Y.) · 2008Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
tmRNA has a dual function as a tRNA and an mRNA to facilitate trans-translation, in which a ribosome can switch between translation of a truncated mRNA and the tmRNA's tag sequence. SmpB is a tmRNA binding protein that has been identified to be essential for trans-translation in vivo. To further study the function of SmpB, an S30 fraction from an Escherichia coli strain, in which the set of genes for SmpB and tmRNA has been deleted from the genome, and His-tagged SmpB active in trans-translation were prepared. The SmpB-depleted S30 fraction had an ability to facilitate poly(U)-dependent tag-peptide synthesis in vitro when purified His-tagged SmpB was exogenously added together with tmRNA, although SmpB was not required for in vitro poly(U)-dependent poly(Phe) synthesis. It was also found that depletion of SmpB leads to a decrease in the level of tmRNA in the cell. In addition, SmpB considerably enhanced the aminoacylation of tmRNA by alanyl-tRNA synthetase in vitro. The aminoacylation enhancement by SmpB, the binding of SmpB to tmRNA and the effect of depletion of SmpB on the expression level of tmRNA in the cell were all affected by some mutations in the tRNA-like domain which cause a defect in ribosome binding leading to a trans-translation deficiency. These results demonstrate that, via binding to the tRNA-like domain of tmRNA, SmpB plays various roles: rescuing the tmRNA molecule from degradation in the cell, enhancing the aminoacylation of tmRNA and mediating the binding of tmRNA to ribosome.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.