ArticleNucleic acids research2002
Rhodobacter sphaeroides LexA has dual activity: optimising and repressing recA gene transcription.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Genome-Wide Identification of the LexA-Mediated DNA Damage Response in Streptomyces venezuelae.Journal of bacteriology · 2022Article
- Non-canonical LexA proteins regulate the SOS response in the Bacteroidetes.Nucleic acids research · 2021Article
- Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus.Nature communications · 2019Article
- Lytic gene expression in the temperate bacteriophage GIL01 is activated by a phage-encoded LexA homologue.Nucleic acids research · 2018Article
- The interaction of ω2 with the RNA polymerase β' subunit functions as an activation to repression switch.Nucleic acids research · 2015Article
- An SOS Regulon under Control of a Noncanonical LexA-Binding Motif in the Betaproteobacteria.Journal of bacteriology · 2015Article
- Involvement of PG2212 zinc finger protein in the regulation of oxidative stress resistance in Porphyromonas gingivalis W83.Journal of bacteriology · 2014Article
- Identification of a DNA-damage-inducible regulon in Acinetobacter baumannii.Journal of bacteriology · 2013Article
- DNA damage responses in prokaryotes: regulating gene expression, modulating growth patterns, and manipulating replication forks.Cold Spring Harbor perspectives in biology · 2013Review
- Global analysis of the regulon of the transcriptional repressor LexA, a key component of SOS response in Mycobacterium tuberculosis.The Journal of biological chemistry · 2012Article
- Vibrio cholerae LexA coordinates CTX prophage gene expression.Journal of bacteriology · 2009Article
- Characterization of the SOS regulon of Caulobacter crescentus.Journal of bacteriology · 2008Article
- Proteomic identification of a novel Anaplasma phagocytophilum DNA binding protein that regulates a putative transcription factor.Journal of bacteriology · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription of the Rhodobacter sphaeroides recA promoter (P(recA)) is induced upon DNA damage in a lexA-dependent manner. In vivo experiments demonstrate that LexA protein represses and might also activate transcription of P(recA). Purified R.sphaeroides LexA protein specifically binds the SOS boxes located within the P(recA) region. In vitro transcription analysis, using Escherichia coli RNA polymerase (RNAP), indicated that the presence of LexA may stimulate and repress transcription of P(recA). EMSA and DNase I footprinting experiments show that LexA and RNAP can bind simultaneously to P(recA). At low LexA concentrations it enhances RNAP binding to P(recA), stimulates open complex formation and strand separation beyond the transcription start site. At high LexA concentrations, however, RNAP-promoted strand separation is not observed beyond the +5 region. LexA might repress transcription by interfering with the clearance process instead of blocking the access of RNAP to the promoter region. Based on these findings we propose that the R.sphaeroides LexA protein performs fine tuning of the SOS response, which might provide a physiological advantage by enhancing transcription of SOS genes and delaying full activation of the response.
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