ArticleBiophysical journal2002
Three-dimensional cellular deformation analysis with a two-photon magnetic manipulator workstation.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 81 citations in OpenAlex.
- Magnetically Driven Hydrogel Surfaces for Modulating Macrophage Behavior.ACS biomaterials science & engineering · 2024Article
- Revisiting tissue tensegrity: Biomaterial-based approaches to measure forces across length scales.APL bioengineering · 2021Review
- Spatial mapping of tissue properties in vivo reveals a 3D stiffness gradient in the mouse limb bud.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- FMSP-Nanoparticles Induced Cell Death on Human Breast Adenocarcinoma Cell Line (MCF-7 Cells): Morphometric Analysis.Biomolecules · 2018Article
- A microfluidic platform reveals differential response of regulatory T cells to micropatterned costimulation arrays.Integrative biology : quantitative biosciences from nano to macro · 2015Article
- Cell mechanosensitivity: mechanical properties and interaction with gravitational field.BioMed research international · 2013Review
- A High-Throughput Technique Reveals the Load- and Site Density-Dependent Kinetics of E-Selectin.Cellular and molecular bioengineering · 2012Article
- Magnetic nanoparticle-mediated massively parallel mechanical modulation of single-cell behavior.Nature methods · 2012Article
- A silicone-based stretchable micropost array membrane for monitoring live-cell subcellular cytoskeletal response.Lab on a chip · 2012Article
- SAM-based cell transfer to photopatterned hydrogels for microengineering vascular-like structures.Biomaterials · 2011Article
- Design and Modeling of a 3-D Magnetic Actuator for Magnetic Microbead Manipulation.IEEE/ASME transactions on mechatronics : a joint publication of the IEEE Industrial Electronics Society and the ASME Dynamic Systems and Control Division · 2011Article
- Magnet polepiece design for uniform magnetic force on superparamagnetic beads.The Review of scientific instruments · 2010Article
- Spatiotemporal measurement of freezing-induced deformation of engineered tissues.Journal of biomechanical engineering · 2010Article
- Nanoscale probing reveals that reduced stiffness of clots from fibrinogen lacking 42 N-terminal Bbeta-chain residues is due to the formation of abnormal oligomers.Biophysical journal · 2009Article
- Agnostic particle tracking for three-dimensional motion of cellular granules and membrane-tethered bead dynamics.Biophysical journal · 2008Article
- 3D particle tracking on a two-photon microscope.Journal of fluorescence · 2006Article
- Thin-foil magnetic force system for high-numerical-aperture microscopy.The Review of scientific instruments · 2006Article
- Micro magnetic tweezers for nanomanipulation inside live cells.Biophysical journal · 2005Article
- A three-dimensional viscoelastic model for cell deformation with experimental verification.Biophysical journal · 2003Article
- Tensile force-dependent neurite elicitation via anti-beta1 integrin antibody-coated magnetic beads.Biophysical journal · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to apply quantifiable mechanical stresses at the microscopic scale is critical for studying cellular responses to mechanical forces. This necessitates the use of force transducers that can apply precisely controlled forces to cells while monitoring the responses noninvasively. This paper describes the development of a micromanipulation workstation integrating two-photon, three-dimensional imaging with a high-force, uniform-gradient magnetic manipulator. The uniform-gradient magnetic field applies nearly uniform forces to a large cell population, permitting statistical quantification of select molecular responses to mechanical stresses. The magnetic transducer design is capable of exerting over 200 pN of force on 4.5-microm-diameter paramagnetic particles and over 800 pN on 5.0-microm ferromagnetic particles. These forces vary within +/-10% over an area 500 x 500 microm2. The compatibility with the use of high numerical aperture (approximately 1.0) objectives is an integral part of the workstation design allowing submicron-resolution, three-dimensional, two-photon imaging. Three-dimensional analyses of cellular deformation under localized mechanical strain are reported. These measurements indicate that the response of cells to large focal stresses may contain three-dimensional global deformations and show the suitability of this workstation to further studying cellular response to mechanical stresses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.