ArticleBiophysical journal2002
Block of the ryanodine receptor channel by neomycin is relieved at high holding potentials.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 29 citations in OpenAlex.
- Calcium channel inhibitor and extracellular calcium improve aminoglycoside-induced hair cell loss in zebrafish.Archives of toxicology · 2024Article
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- Acute Induction of Translocon-Mediated CaCells · 2020Article
- Protecting Mammalian Hair Cells from Aminoglycoside-Toxicity: Assessing Phenoxybenzamine's Potential.Frontiers in cellular neuroscience · 2017Article
- Inhibition of peripheral nociceptors by aminoglycosides produces analgesia in inflammatory pain models in the rat.Inflammation · 2015Article
- Selecting ions by size in a calcium channel: the ryanodine receptor case study.Biophysical journal · 2014Article
- Article
- The contribution of hydrophobic residues in the pore-forming region of the ryanodine receptor channel to block by large tetraalkylammonium cations and Shaker B inactivation peptides.The Journal of general physiology · 2012Article
- Subtype identification and functional characterization of ryanodine receptors in rat cerebral artery myocytes.American journal of physiology. Cell physiology · 2010Article
- Glucagon-like peptide-1 increases myocardial glucose uptake via p38alpha MAP kinase-mediated, nitric oxide-dependent mechanisms in conscious dogs with dilated cardiomyopathy.Circulation. Heart failure · 2010 · on this mapArticle
- Article
- Changes in negative charge at the luminal mouth of the pore alter ion handling and gating in the cardiac ryanodine-receptor.Biophysical journal · 2009Article
- Ryanoids and imperatoxin affect the modulation of cardiac ryanodine receptors by dihydropyridine receptor Peptide A.Biochimica et biophysica acta · 2008Article
- The ryanodine receptor pore blocker neomycin also inhibits channel activity via a previously undescribed high-affinity Ca(2+) binding site.The Journal of membrane biology · 2007Article
- On the interaction of neomycin with the slow vacuolar channel of Arabidopsis thaliana.The Journal of general physiology · 2006Article
- Two rings of negative charges in the cytosolic vestibule of type-1 ryanodine receptor modulate ion fluxes.Biophysical journal · 2006Article
- The aminoglycoside antibiotic dihydrostreptomycin rapidly enters mouse outer hair cells through the mechano-electrical transducer channels.The Journal of physiology · 2005Article
- Electrostatic mechanisms underlie neomycin block of the cardiac ryanodine receptor channel (RyR2).Biophysical journal · 2004Article
- A model of the putative pore region of the cardiac ryanodine receptor channel.Biophysical journal · 2004Article
- Evidence for the transport of glutathione through ryanodine receptor channel type 1.The Biochemical journal · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study we have investigated the actions of the aminoglycoside antibiotic neomycin on K+ conductance in the purified sheep cardiac sarcoplasmic reticulum (SR) calcium-release channel (RyR). Neomycin induces a concentration- and voltage-dependent partial block from both the cytosolic and luminal faces of the channel. Blocking parameters for cytosolic and luminal block are markedly different. Neomycin has a greater affinity for the luminal site of interaction than the cytosolic site: zero-voltage dissociation constants (Kb(0)) are respectively 210.20 +/- 22.80 and 589.70 +/- 184.00 nM for luminal and cytosolic block. However, neomycin also exhibits voltage-dependent relief of block at holding potentials >+60 mV when applied to the cytosolic face and a similar phenomenon may occur with luminal neomycin at high negative holding potentials. These observations indicate that, under appropriate conditions, neomycin is capable of passing through the RyR channel.
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