ArticleBiophysical journal2002
Stoichiometry of the Cardiac Na+/Ca2+ exchanger NCX1.1 measured in transfected HEK cells.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 78 citations in OpenAlex.
- A mechanism of lysosomal calcium entry.Science advances · 2024Article
- Structural insight into the allosteric inhibition of human sodium-calcium exchanger NCX1 by XIP and SEA0400.The EMBO journal · 2024Article
- Insights into the molecular basis of the palmitoylation and depalmitoylation of NCX1.Cell calcium · 2021Article
- Peptide hormone sensors using human hormone receptor-carrying nanovesicles and graphene FETs.Scientific reports · 2020Article
- Sodium recognition by the Na+/Ca2+ exchanger in the outward-facing conformation.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A novel delta current method for transport stoichiometry estimation.BMC biophysics · 2014Article
- The Concise Guide to PHARMACOLOGY 2013/14: transporters.British journal of pharmacology · 2013Article
- Structural arrangement of the intracellular Ca2+ binding domains of the cardiac Na+/Ca2+ exchanger (NCX1.1): effects of Ca2+ binding.The Journal of biological chemistry · 2013Article
- Characterization and purification of a Na+/Ca2+ exchanger from an archaebacterium.The Journal of biological chemistry · 2012Article
- Brassica juncea plant cadmium resistance 1 protein (BjPCR1) facilitates the radial transport of calcium in the root.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Simulation of the undiseased human cardiac ventricular action potential: model formulation and experimental validation.PLoS computational biology · 2011Article
- A transmural gradient in the cardiac Na/K pump generates a transmural gradient in Na/Ca exchange.The Journal of membrane biology · 2010Article
- Involvement of Na+/Ca2+ exchanger in migration and contraction of rat cultured tendon fibroblasts.The Journal of physiology · 2009Article
- Sodium-calcium exchange mediated contraction in left anterior descending and left ventricular branch arteries.Journal of cellular and molecular medicine · 2009Article
- Phospholemman regulates cardiac Na+/Ca2+ exchanger by interacting with the exchanger's proximal linker domain.American journal of physiology. Cell physiology · 2009Article
- The sodium-calcium exchanger is a mechanosensitive transporter.The Journal of physiology · 2008Article
- Metabolic regulation of sodium-calcium exchange by intracellular acyl CoAs.The EMBO journal · 2006Article
- Separate ion pathways in a Cl-/H+ exchanger.The Journal of general physiology · 2005Article
- Allosteric activation of sodium-calcium exchange activity by calcium: persistence at low calcium concentrations.The Journal of general physiology · 2003Article
- Resting membrane potential regulates Na(+)-Ca2+ exchange-mediated Ca2+ overload during hypoxia-reoxygenation in rat ventricular myocytes.The Journal of physiology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The stoichiometry with which the Na+/Ca2+ exchanger, NCX1, binds and transports Na+ and Ca2+ has dramatic consequences for ionic homeostasis and cellular function of heart mycocytes and brain neurons, where the exchanger is highly expressed. Previous studies have examined this question using native NCX1 in its endogenous environment. We describe here whole-cell voltage clamp studies using recombinant rat heart NCX1.1 expressed heterologously in HEK-293 cells. This system provides the advantages of a high level of NCX1 protein expression, very low background ion transport levels, and excellent control over clamped voltage and ionic composition. Using ionic conditions that allowed bi-directional currents, voltage ramps were employed to determine the reversal potential for NCX1.1-mediated currents. Analysis of the relation between reversal potential and external [Na+] or [Ca2+], under a variety of intracellular conditions, yielded coupling ratios for Na+ of 1.9-2.3 ions per net charge and for Ca2+ of 0.45 +/- 0.03 ions per net charge. These data are consistent with a stoichiometry for the NCX1.1 protein of 4 Na+ to 1 Ca2+ to 2 charges moved per transport cycle.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.