ArticleBiophysical journal2002
Protein kinase C phosphorylation of purified Na,K-ATPase: C-terminal phosphorylation sites at the alpha- and gamma-subunits close to the inner face of the plasma membrane.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 33 citations in OpenAlex.
- Regulation of Neuronal NaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Distinct pH dependencies of NaThe Journal of biological chemistry · 2018Article
- Electrostatic Stabilization Plays a Central Role in Autoinhibitory Regulation of the NaBiophysical journal · 2017Article
- Distinct α2 Na,K-ATPase membrane pools are differently involved in early skeletal muscle remodeling during disuse.The Journal of general physiology · 2016Article
- Phosphorylation of rat kidney Na-K pump at Ser938 is required for rapid angiotensin II-dependent stimulation of activity and trafficking in proximal tubule cells.American journal of physiology. Cell physiology · 2016Article
- Isoform-specific Na,K-ATPase alterations precede disuse-induced atrophy of rat soleus muscle.BioMed research international · 2015Article
- Angiotensin II-dependent phosphorylation at Ser11/Ser18 and Ser938 shifts the E2 conformations of rat kidney Na+/K+-ATPase.The Biochemical journal · 2012Article
- Ovariectomy alters energy metabolism in rat striatum: effect of supplementation with soy diet rich in isoflavones.Metabolic brain disease · 2011Article
- Modulation of protein kinase C by curcumin; inhibition and activation switched by calcium ions.British journal of pharmacology · 2007Article
- Curcumin modulation of Na,K-ATPase: phosphoenzyme accumulation, decreased K+ occlusion, and inhibition of hydrolytic activity.British journal of pharmacology · 2005Article
- Direct activation of gastric H,K-ATPase by N-terminal protein kinase C phosphorylation. Comparison of the acute regulation mechanisms of H,K-ATPase and Na,K-ATPase.Biophysical journal · 2003Article
- Red blood cell Na pump: Insights from species differences.Blood cells, molecules & diseasesReview
- S163 is critical for FXYD5 modulation of wound healing in airway epithelial cells.Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair SocietyArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The alpha-subunit of the Na,K-ATPase is phosphorylated at specific sites by protein kinases A and C. Phosphorylation by protein kinase C (PKC) is restricted to the N terminus and takes place to a low stoichiometry, except in rat. Here we show that the alpha-subunit of shark Na,K-ATPase can be phosphorylated by PKC at C-terminal sites to stoichiometric levels in the presence of detergents. Two novel phosphorylation sites are possible candidates for this PKC phosphorylation: Thr-938 in the M8/M9 loop located very close to the PKA site, and Ser-774, in the proximal part of the M5/M6 hairpin. Both sites are highly conserved in all known alpha-subunits, indicating a physiological role. A similar pattern of detergent-mediated phosphorylation by PKC was found in pig kidney Na,K-ATPase alpha-subunit. Interestingly, the kidney-specific gamma-subunit was phosphorylated by PKC in the presence of detergent. The close proximity of the novel PKC sites to the membrane suggests that targeting proteins to tether PKC into the membrane phase is important in controlling the in vivo phosphorylation of this novel class of membrane-adjacent PKC sites. It is suggested that in purified preparations where functional targeting may be impaired detergents are needed to expose the sites.
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