ArticleBiophysical journal2002
MYO1A (brush border myosin I) dynamics in the brush border of LLC-PK1-CL4 cells.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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Who cites it
79 citing papers in PubMed, 135 citations in OpenAlex.
- Myosin-9b Controls Epithelial Brush Border Architecture through Motility-Dependent RhoA Signaling.bioRxiv : the preprint server for biology · 2026Article
- The Genetic Architecture of Congenital Diarrhea and Enteropathy.The New England journal of medicine · 2025Article
- The microvillar protocadherin CDHR5 associates with EBP50 to promote brush border assembly.Molecular biology of the cell · 2024Article
- The morphological and functional diversity of apical microvilli.Journal of anatomy · 2023Review
- Building the brush border, one microvillus at a time.Current opinion in cell biology · 2023Review
- Manipulation of microvillar proteins duringFrontiers in cellular and infection microbiology · 2023Article
- Mitotic Spindle Positioning (MISP) is an actin bundler that selectively stabilizes the rootlets of epithelial microvilli.Cell reports · 2022Article
- Deafness mutation in the MYO3A motor domain impairs actin protrusion elongation mechanism.Molecular biology of the cell · 2022Article
- A protocol for imaging microvilli biogenesis on the surface of cultured porcine kidney epithelial cell monolayers.STAR protocols · 2021Article
- Stochastic contraction of myosin minifilaments drives evolution of microridge protrusion patterns in epithelial cells.Molecular biology of the cell · 2021Article
- Actin at stereocilia tips is regulated by mechanotransduction and ADF/cofilin.Current biology : CB · 2021Article
- Functional Role of Class III Myosins in Hair Cells.Frontiers in cell and developmental biology · 2021Review
- Nonmuscle myosin-2 contractility-dependent actin turnover limits the length of epithelial microvilli.Molecular biology of the cell · 2020Article
- Keratins and the plakin family cytolinker proteins control the length of epithelial microridge protrusions.eLife · 2020Article
- The small EF-hand protein CALML4 functions as a critical myosin light chain within the intermicrovillar adhesion complex.The Journal of biological chemistry · 2020Article
- Myosin 1a Regulates Osteoblast Differentiation Independent of Intestinal Calcium Transport.Journal of the Endocrine Society · 2019Article
- Profilin-Mediated Actin Allocation Regulates the Growth of Epithelial Microvilli.Current biology : CB · 2019Article
- Actin Dynamics Drive Microvillar Motility and Clustering during Brush Border Assembly.Developmental cell · 2019Article
- Identification of Novel PTPRQ and MYO1A Mutations in An Iranian Pedigree with Autosomal Recessive Hearing Loss.Cell journal · 2018Article
- MyTH4-FERM myosins in the assembly and maintenance of actin-based protrusions.Current opinion in cell biology · 2017Review
19 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
The kidney epithelial cell line, LLC-PK1-CL4 (CL4), forms a well ordered brush border (BB) on its apical surface. CL4 cells were used to examine the dynamics of MYO1A (M1A; formerly BB myosin I) within the BB using GFP-tagged MIA (GFP-M1A), MIA motor domain (GFP-MDIQ), and tail domain (GFP-Tail). GFP-beta-actin (GFP-Actin) was used to assess actin dynamics within the BB. GFP-M1A, GFP-Tail, but not GFP-MDIQ localized to the BB, indicating that the tail is sufficient for apical targeting of M1A. GFP-Actin targeted to all the actin domains of the cell including the BB. Fluorescence recovery after photobleaching analysis revealed that GFP-M1A and GFP-Tail turnover in the BB is rapid, approximately 80% complete in <1 min. As expected for an actin-based motor, ATP depletion resulted in significant inhibition of GFP-M1A turnover yet had little effect on GFP-Tail exchange. Rapid turnover of GFP-M1A and GFP-Tail was not due to actin turnover as GFP-Actin turnover in the BB was much slower. These results indicate that the BB population of M1A turns over rapidly, while its head and tail domains interact transiently with the core actin and plasma membrane, respectively. This rapidly exchanging pool of M1A envelops an actin core bundle that, by comparison, is static in structure.
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