ArticleBiophysical journal2002
Kinetics and mechanism of intercellular ice propagation in a micropatterned tissue construct.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 77 citations in OpenAlex.
- Automated image analysis for intracellular ice detection in monolayers by freeze-substitution.Npj imaging · 2026Article
- A three-dimensional lattice-free agent-based model of intracellular ice formation and propagation and intercellular mechanics in liver tissues.Royal Society open science · 2024Article
- Cryopreservation of tissues by slow-freezing using an emerging zwitterionic cryoprotectant.Scientific reports · 2023Article
- Cryopreservation of human cerebral microvascular endothelial cells and astrocytes in suspension and monolayers.PloS one · 2021Article
- Extracellular Antifreeze Protein Significantly Enhances the Cryopreservation of Cell Monolayers.Biomacromolecules · 2019Article
- Freezing Responses in DMSO-Based Cryopreservation of Human iPS Cells: Aggregates Versus Single Cells.Tissue engineering. Part C, Methods · 2018Article
- Directional freezing for the cryopreservation of adherent mammalian cells on a substrate.PloS one · 2018Article
- Intracellular ice formation: the enigmatic role of cell-cell junctions.Biophysical journal · 2013Article
- Effects of intercellular junction protein expression on intracellular ice formation in mouse insulinoma cells.Biophysical journal · 2013Article
- Effect of the expression of aquaporins 1 and 3 in mouse oocytes and compacted eight-cell embryos on the nucleation temperature for intracellular ice formation.Reproduction (Cambridge, England) · 2011Article
- Stability of mouse oocytes at -80 °C: the role of the recrystallization of intracellular ice.Reproduction (Cambridge, England) · 2011Article
- Thermostability of biological systems: fundamentals, challenges, and quantification.The open biomedical engineering journal · 2011Article
- Cryopreservation of Human Stem Cells for Clinical Application: A Review.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2011Article
- Intracellular ice formation in confluent monolayers of human dental stem cells and membrane damage.Cryobiology · 2010Article
- The temperature and type of intracellular ice formation in preimplantation mouse embryos as a function of the developmental stage.Biology of reproduction · 2010Article
- A biologist's view of the relevance of thermodynamics and physical chemistry to cryobiology.Cryobiology · 2010Review
- Structural coupling of cardiomyocytes and noncardiomyocytes: quantitative comparisons using a novel micropatterned cell pair assay.American journal of physiology. Heart and circulatory physiology · 2008Article
- Engineering complex tissues.Tissue engineering · 2006Review
- Kinetics of intracellular ice formation in one-dimensional arrays of interacting biological cells.Biophysical journal · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding the effects of cell-cell interaction on intracellular ice formation (IIF) is required to design optimized protocols for cryopreservation of tissue. To determine the effects of cell-cell interactions during tissue freezing, without confounding effects from uncontrolled factors (such as time in culture, cell geometry, and cell-substrate interactions), HepG2 cells were cultured in pairs on glass coverslips micropatterned with polyethylene glycol disilane, such that each cell interacted with exactly one adjacent cell. Assuming the cell pair to be a finite state system, being either in an unfrozen state (no ice in either cell), a singlet state (IIF in one cell only), or a doublet state (IIF in both cells), the kinetics of state transitions were theoretically modeled and cryomicroscopically measured. The rate of intercellular ice propagation, estimated from the measured singlet state probability, increased in the first 24 h of culture and remained steady thereafter. In cell pairs cultured for 24 h and treated with the gap junction blocker 18beta-glycyrrhetinic acid before freezing, the intercellular ice propagation rate was lower than in untreated controls (p < 0.001), but significantly greater than zero (p < 0.0001). These results suggest that gap junctions mediate some, but not all, mechanisms of ice propagation in tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.