ArticleBiophysical journal2002
Measurement of the lateral diffusion of human MHC class I molecules on HeLa cells by fluorescence recovery after photobleaching using a phycoerythrin probe.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Biophysical basis underlying dynamic Lck activation visualized by ZapLck FRET biosensor.Science advances · 2019Article
- Transmembrane domain dependent inhibitory function of FcγRIIB.Protein & cell · 2018Review
- Mapping diffusion in a living cell via the phasor approach.Biophysical journal · 2014Article
- The effect of ligand affinity on integrins' lateral diffusion in cultured cells.European biophysics journal : EBJ · 2013Article
- Proposed correction to Feder's anomalous diffusion FRAP equations.Biophysical journal · 2011Article
- A mathematical framework for analyzing T cell receptor scanning of peptides.Biophysical journal · 2010Article
- HIV-1 Nef binds a subpopulation of MHC-I throughout its trafficking itinerary and down-regulates MHC-I by perturbing both anterograde and retrograde trafficking.The Journal of biological chemistry · 2010Article
- Membrane mobility of beta2 integrins and rolling associated adhesion molecules in resting neutrophils.Biophysical journal · 2008Article
- Increased mobility of major histocompatibility complex I-peptide complexes decreases the sensitivity of antigen recognition.The Journal of biological chemistry · 2008Article
- Fluorescence recovery under decaying photobleaching irradiation: concept and experiment.Journal of fluorescence · 2006Article
- Short class I major histocompatibility complex cytoplasmic tails differing in charge detect arbiters of lateral diffusion in the plasma membrane.Biophysical journal · 2004Article
- Lowering the barriers to random walks on the cell surface.Biophysical journal · 2003Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mobility of cell surface MHC class I molecules on HeLa cells was measured by fluorescence recovery after photobleaching (FRAP). The probe used for these studies was the phycobiliprotein R-phycoerythrin coupled to Fab fragments of a monoclonal antibody specific for human monomorphic MHC class I molecules. It was found that the recovery curves could be equally well fitted by either a random diffusion model with an immobile component or by an anomalous diffusion model. In the latter case, the anomalous diffusion exponent was consistent with that previously determined by single-particle tracking (SPT) experiments using the same probe (P. R. Smith, I. E. G. Morrison, K. M. Wilson, N. Fernandez, and R. J. Cherry. 1999. Biophys. J. 76:3331-3344). The FRAP experiments, however, yielded a considerably higher value of D(0), the diffusion coefficient for a time interval of 1 s. To determine whether the results were probe dependent, FRAP measurements were also performed with the same monoclonal antibody labeled with Oregon Green. These experiments gave similar results to those obtained with the phycoerythrin probe. FRAP experiments with the lipid probe 5-N-(octadecanoyl) aminofluoroscein (ODAF) bound to HeLa cells gave typical results for lipid diffusion. Overall, our observations and analysis are consistent with anomalous diffusion of MHC class I diffusion on HeLa cells, but quantitative differences between FRAP and SPT data remain to be explained.
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