ArticleApplied and environmental microbiology2002
Reduced oxidative pentose phosphate pathway flux in recombinant xylose-utilizing Saccharomyces cerevisiae strains improves the ethanol yield from xylose.
Article in Applied and environmental microbiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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54 citing papers in PubMed, 185 citations in OpenAlex.
- Antifungal activity of the culture filtrate ofFrontiers in microbiology · 2026Article
- Recent progress in engineering yeast producers of cellulosic ethanol.FEMS yeast research · 2025Review
- Review
- Exploitation of Hetero- and Phototrophic Metabolic Modules for Redox-Intensive Whole-Cell Biocatalysis.Frontiers in bioengineering and biotechnology · 2022Review
- Transcription Analysis of RecombinantJournal of microbiology and biotechnology · 2020Article
- Investigation of Heterologously Expressed Glucose-6-Phosphate Dehydrogenase Genes in a YeastMicroorganisms · 2020Article
- Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha).Journal of industrial microbiology & biotechnology · 2020Review
- Pentose Metabolism in Saccharomyces cerevisiae: The Need to Engineer Global Regulatory Systems.Biotechnology journal · 2019Review
- Effect of nitrogen availability on the poly-3-D-hydroxybutyrate accumulation by engineered Saccharomyces cerevisiae.AMB Express · 2017Article
- Production of fuels and chemicals from xylose by engineered Saccharomyces cerevisiae: a review and perspective.Microbial cell factories · 2017Review
- Article
- A design-build-test cycle using modeling and experiments reveals interdependencies between upper glycolysis and xylose uptake in recombinantBiotechnology for biofuels · 2017Article
- Accumulation of metabolic side products might favor the production of ethanol in Pho13 knockout strains.Microbial cell (Graz, Austria) · 2016Article
- Article
- Exploring xylose metabolism in Spathaspora species: XYL1.2 from Spathaspora passalidarum as the key for efficient anaerobic xylose fermentation in metabolic engineered Saccharomyces cerevisiae.Biotechnology for biofuels · 2016Article
- Review
- Influence of the propagation strategy for obtaining robust Saccharomyces cerevisiae cells that efficiently co-ferment xylose and glucose in lignocellulosic hydrolysates.Microbial biotechnology · 2015Article
- The return of metabolism: biochemistry and physiology of the pentose phosphate pathway.Biological reviews of the Cambridge Philosophical Society · 2015Review
- Metabolic engineering of strains: from industrial-scale to lab-scale chemical production.Journal of industrial microbiology & biotechnology · 2015Review
- Identification of novel metabolic interactions controlling carbon flux from xylose to ethanol in natural and recombinant yeasts.Biotechnology for biofuels · 2015Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In recombinant, xylose-fermenting Saccharomyces cerevisiae, about 30% of the consumed xylose is converted to xylitol. Xylitol production results from a cofactor imbalance, since xylose reductase uses both NADPH and NADH, while xylitol dehydrogenase uses only NAD(+). In this study we increased the ethanol yield and decreased the xylitol yield by lowering the flux through the NADPH-producing pentose phosphate pathway. The pentose phosphate pathway was blocked either by disruption of the GND1 gene, one of the isogenes of 6-phosphogluconate dehydrogenase, or by disruption of the ZWF1 gene, which encodes glucose 6-phosphate dehydrogenase. Decreasing the phosphoglucose isomerase activity by 90% also lowered the pentose phosphate pathway flux. These modifications all resulted in lower xylitol yield and higher ethanol yield than in the control strains. TMB3255, carrying a disruption of ZWF1, gave the highest ethanol yield (0.41 g g(-1)) and the lowest xylitol yield (0.05 g g(-1)) reported for a xylose-fermenting recombinant S. cerevisiae strain, but also an 84% lower xylose consumption rate. The low xylose fermentation rate is probably due to limited NADPH-mediated xylose reduction. Metabolic flux modeling of TMB3255 confirmed that the NADPH-producing pentose phosphate pathway was blocked and that xylose reduction was mediated only by NADH, leading to a lower rate of xylose consumption. These results indicate that xylitol production is strongly connected to the flux through the oxidative part of the pentose phosphate pathway.
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