ArticleJournal of bacteriology2002
Roles of the C-terminal end of SecY in protein translocation and viability of Escherichia coli.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Substrate-induced assembly and functional mechanism of the membrane protein insertase SecYEG-YidC.The EMBO journal · 2026Article
- Identification and characterization of a translation arrest motif in VemP by systematic mutational analysis.The Journal of biological chemistry · 2018Article
- Alignment of the protein substrate hairpin along the SecA two-helix finger primes protein transport inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Ancestral and derived protein import pathways in the mitochondrion of Reclinomonas americana.Molecular biology and evolution · 2011Article
- Cryo-EM structure of the ribosome-SecYE complex in the membrane environment.Nature structural & molecular biology · 2011Article
- Mapping of the SecA·SecY and SecA·SecG interfaces by site-directed in vivo photocross-linking.The Journal of biological chemistry · 2011Article
- Regulation of the protein-conducting channel by a bound ribosome.Structure (London, England : 1993) · 2009Article
- Article
- Assembly of the translocase motor onto the preprotein-conducting channel.Molecular microbiology · 2008Article
- Oligomeric states of the SecA and SecYEG core components of the bacterial Sec translocon.Biochimica et biophysica acta · 2007Review
- Different modes of SecY-SecA interactions revealed by site-directed in vivo photo-cross-linking.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Genes of Helicobacter pylori regulated by attachment to AGS cells.Infection and immunity · 2004Article
- Importance of transmembrane segments in Escherichia coli SecY.Molecular genetics and genomics : MGG · 2003Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SecY, a central component of the membrane-embedded sector of protein translocase, contains six cytosolic domains. Here, we examined the importance of the C-terminal cytosolic region of SecY by systematically shortening the C-terminal end and examining the functional consequences of these mutations in vivo and in vitro. It was indicated that the C-terminal five residues are dispensable without any appreciable functional defects in SecY. Mutants missing the C-terminal six to seven residues were partially compromised, especially at low temperature or in the absence of SecG. In vitro analyses indicated that the initial phase of the translocation reaction, in which the signal sequence region of the preprotein is inserted into the membrane, was affected by the lack of the C-terminal residues. SecA binding was normal, but SecA insertion in response to ATP and a preprotein was impaired. It is suggested that the C-terminal SecY residues are required for SecA-dependent translocation initiation.
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