ArticleJournal of bacteriology2002
Mutations in the 16S rRNA genes of Helicobacter pylori mediate resistance to tetracycline.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed.
- Molecular Detection Technologies for Helicobacter pylori Antibiotic Resistance: Current Status and Clinical Implications.Current microbiology · 2026Review
- Evolution, Mechanisms, and Therapeutic Implications of Mobile Tetracycline Destructases.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Parental Tetracycline Exposure Induces Genotoxic Damage and Fitness Deficits in Daphnia magna Offspring.Environmental and molecular mutagenesis · 2026Article
- Low-Abundance and FragmentaryBiomolecules · 2026Article
- Lineage-specificbioRxiv : the preprint server for biology · 2026Article
- Evolution and characteristics of tetracycline resistance inMicrobiology spectrum · 2025Article
- Antibiotic Resistance Identification Among the Patients withMiddle East journal of digestive diseases · 2025Article
- Antibiotic resistance in Helicobacter pylori: a genetic and physiological perspective.Gut pathogens · 2025Review
- Overcoming antibiotic-resistantWorld journal of gastroenterology · 2025Review
- Molecular Characterization of Antimicrobial Resistance and Virulence Genotyping amongInfectious disorders drug targets · 2025Article
- Copper enhances tetracycline resistance via the efflux transporter CrdAB-CzcBA inFrontiers in medicine · 2025Article
- Putative mutations associated with tetracycline resistance detected in Treponema spp.: an analysis of 4,355 Spirochaetales genomes.Access microbiology · 2025Article
- Antibiotic Resistance ofJournal of Korean medical science · 2024Review
- Antibiotic Resistance, Susceptibility Testing and Stewardship inInternational journal of molecular sciences · 2023Review
- Antibiotic resistance in Helicobacter pylori: From potential biomolecular mechanisms to clinical practice.Journal of clinical laboratory analysis · 2023Review
- TheFrontiers in microbiology · 2023Article
- Clinical Implication of Drug Resistance forAntibiotics (Basel, Switzerland) · 2022Review
- Helicobacter pylori Infection, Its Laboratory Diagnosis, and Antimicrobial Resistance: a Perspective of Clinical Relevance.Clinical microbiology reviews · 2022Review
- A Survey ofAntimicrobial agents and chemotherapy · 2022Article
- Impact of 16S rRNA Single Nucleotide Polymorphisms on Mycoplasma genitalium Organism Load with Doxycycline Treatment.Antimicrobial agents and chemotherapy · 2022Article
11 more citing papers are in PubMed but not listed here.
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Low-cost and rescue treatments for Helicobacter pylori infections involve combinations of several drugs including tetracycline. Resistance to tetracycline has recently emerged in H. pylori. The 16S rRNA gene sequences of two tetracycline-resistant clinical isolates (MIC = 64 microg/ml) were determined and compared to the consensus H. pylori 16S rRNA sequence. One isolate had four nucleotide substitutions, and the other had four substitutions and two deletions. Natural transformation with the 16S rRNA genes from the resistant organisms conferred tetracycline resistance on susceptible strains. 16S rRNA genes containing the individual mutations were constructed and tested for the ability to confer resistance. Only the 16S rRNA gene containing the triple mutation, AGA965-967TTC, was able to confer tetracycline resistance on H. pylori 26695. The MICs of tetracycline for the transformed strains were equivalent to those for the original clinical isolates. The two original isolates were also metronidazole resistant, but this trait was not linked to the tetracycline resistance phenotype. Serial passage of several H. pylori strains on increasing concentrations of tetracycline yielded mutants with only a very modest increase in tetracycline resistance to a MIC of 4 to 8 microg/ml. These mutants all had a deletion of G942 in the 16S rRNA genes. The mutations in the 16S rRNA are clearly responsible for tetracycline resistance in H. pylori.
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