ArticleGenes & development2002
Subcellular localization of the yeast proteome.
Article in Genes & development, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 276 papers.
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Who cites it
276 citing papers in PubMed.
- A proteome-driven strategy to discover novel therapeutic targets in Orientia tsutsugamushi.Antonie van Leeuwenhoek · 2026Article
- Dynamic Organellar Mapping in yeast reveals extensive protein localization changes during ER stress.Nature communications · 2025Article
- Strangers in a foreign land: 'Yeastizing' plant enzymes.Microbial biotechnology · 2024Review
- Thermal Stress and Nuclear Transport.Advances in experimental medicine and biology · 2024Review
- Fresh Insights IntoOncology reviews · 2024Review
- Discovery of a novel marker for human granulocytes and tissue macrophages: RTL1 revisited.Cell and tissue research · 2023Article
- Practical Approaches for the YeastInternational journal of molecular sciences · 2023Review
- Unraveling the Secrets of a Double-Life Fungus by Genomics:Journal of fungi (Basel, Switzerland) · 2023Article
- Advances in transposable elements: from mechanisms to applications in mammalian genomics.Frontiers in genetics · 2023Review
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- The development and application of matrix assisted laser desorption electrospray ionization: The teenage years.Mass spectrometry reviews · 2023Review
- Protein fluorescent labeling in live yeast cells using scFv-based probes.Cell reports methods · 2022Article
- Determining protein polarization proteome-wide using physical dissection of individual Stentor coeruleus cells.Current biology : CB · 2022Article
- DNA circles promote yeast ageing in part through stimulating the reorganization of nuclear pore complexes.eLife · 2022Article
- Genetic and Physiological Characterization of Fructose-1,6-Bisphosphate Aldolase and Glyceraldehyde-3-Phosphate Dehydrogenase in the Crabtree-Negative YeastInternational journal of molecular sciences · 2022Article
- Set1-mediated H3K4 methylation is required forVirulence · 2021Article
- Learning from Yeast about Mitochondrial Carriers.Microorganisms · 2021Review
- An iteration model for identifying essential proteins by combining comprehensive PPI network with biological information.BMC bioinformatics · 2021Article
- Dynamic post-transcriptional regulation by Mrn1 links cell wall homeostasis to mitochondrial structure and function.PLoS genetics · 2021Article
- Imaging-based screens of pool-synthesized cell libraries.Nature methods · 2021Review
216 more citing papers are in PubMed but not listed here.
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Authors and funding
15 authors.
Funding
Abstract
Protein localization data are a valuable information resource helpful in elucidating eukaryotic protein function. Here, we report the first proteome-scale analysis of protein localization within any eukaryote. Using directed topoisomerase I-mediated cloning strategies and genome-wide transposon mutagenesis, we have epitope-tagged 60% of the Saccharomyces cerevisiae proteome. By high-throughput immunolocalization of tagged gene products, we have determined the subcellular localization of 2744 yeast proteins. Extrapolating these data through a computational algorithm employing Bayesian formalism, we define the yeast localizome (the subcellular distribution of all 6100 yeast proteins). We estimate the yeast proteome to encompass approximately 5100 soluble proteins and >1000 transmembrane proteins. Our results indicate that 47% of yeast proteins are cytoplasmic, 13% mitochondrial, 13% exocytic (including proteins of the endoplasmic reticulum and secretory vesicles), and 27% nuclear/nucleolar. A subset of nuclear proteins was further analyzed by immunolocalization using surface-spread preparations of meiotic chromosomes. Of these proteins, 38% were found associated with chromosomal DNA. As determined from phenotypic analyses of nuclear proteins, 34% are essential for spore viability--a percentage nearly twice as great as that observed for the proteome as a whole. In total, this study presents experimentally derived localization data for 955 proteins of previously unknown function: nearly half of all functionally uncharacterized proteins in yeast. To facilitate access to these data, we provide a searchable database featuring 2900 fluorescent micrographs at http://ygac.med.yale.edu.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.