ArticleBMC neuroscience2002
Subunit modification and association in VR1 ion channels.
Article in BMC neuroscience, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Review
- Anti-Inflammatory Effect of Beta-Caryophyllene Mediated by the Involvement of TRPV1, BDNF and trkB in the Rat Cerebral Cortex after Hypoperfusion/Reperfusion.International journal of molecular sciences · 2022Article
- A review of non-prostanoid, eicosanoid receptors: expression, characterization, regulation, and mechanism of action.Journal of cell communication and signaling · 2022Review
- TRPV1 channels and the progesterone receptor Sig-1R interact to regulate pain.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- TRPV1 receptor in the human trigeminal ganglion and spinal nucleus: immunohistochemical localization and comparison with the neuropeptides CGRP and SP.Journal of anatomy · 2016Article
- Bortezomib treatment produces nocifensive behavior and changes in the expression of TRPV1, CGRP, and substance P in the rat DRG, spinal cord, and sciatic nerve.BioMed research international · 2014Article
- High-resolution views of TRPV1 and their implications for the TRP channel superfamily.Handbook of experimental pharmacology · 2014Review
- Distinct properties of Ca2+-calmodulin binding to N- and C-terminal regulatory regions of the TRPV1 channel.The Journal of general physiology · 2012Article
- Antioncogenic effects of transient receptor potential vanilloid 1 in the progression of transitional urothelial cancer of human bladder.ISRN urology · 2012Article
- The endoplasmic reticulum of dorsal root ganglion neurons contains functional TRPV1 channels.The Journal of biological chemistry · 2009Article
- Proton inhibition of unitary currents of vanilloid receptors.The Journal of general physiology · 2009Article
- Rapid, opioid-sensitive mechanisms involved in transient receptor potential vanilloid 1 sensitization.The Journal of biological chemistry · 2008Article
- Functional characterisation of the S512Y mutant vanilloid human TRPV1 receptor.British journal of pharmacology · 2005Article
- Clues to understanding cold sensation: thermodynamics and electrophysiological analysis of the cold receptor TRPM8.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- A TRPV2-PKA signaling module for transduction of physical stimuli in mast cells.The Journal of experimental medicine · 2004Article
- Activation and sensitisation of the vanilloid receptor: role in gastrointestinal inflammation and function.British journal of pharmacology · 2004Review
- State-dependent block of CNG channels by dequalinium.The Journal of general physiology · 2004Article
- Ca2+/calmodulin modulates TRPV1 activation by capsaicin.The Journal of general physiology · 2004Article
- Capsaicin activation of the pain receptor, VR1: multiple open states from both partial and full binding.Biophysical journal · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe capsaicin (vanilloid) receptor, VR1, is an agonist-activated ion channel expressed by sensory neurons that serves as a detector of chemical and thermal noxious stimuli.
resultsIn the present study we investigated the properties of VR1 ion channels expressed in Xenopus oocytes. A VR1 subunit with a FLAG epitope tag at the C-terminus was constructed. When examined for size on an SDS gel, VR1-expressing oocytes produced a doublet corresponding to the size of the monomer and a band at about twice the molecular weight of the monomer. A consensus site for N-linked glycosylation was identified in the primary sequence at position 604. In channels in which the putative glycosylation site was mutated from asparagine to serine (N604S), the larger of the two monomer bands could no longer be detected on the gel. Electrophysiological experiments showed these unglycosylated channels to be functional. The high molecular weight band observed on the gel could represent either a dimer or a monomer conjugated to an unknown factor. To distinguish between these possibilities, we coexpressed a truncated VR1 subunit with full-length VR1. A band of intermediate molecular weight (composed of one full-length and one truncated subunit) was observed. This dimer persisted under strongly reducing conditions, was not affected by capsaicin or calcium, and was refractory to treatment with transglutaminase inhibitors.
conclusionsThe persistence of this dimer even under harsh denaturing and reducing conditions indicates a strong interaction among pairs of subunits. This biochemical dimerization is particularly intriguing given that functional channels are almost certainly tetramers.
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