ArticleBMC cell biology2002
The new anti-actin agent dihydrohalichondramide reveals fenestrae-forming centers in hepatic endothelial cells.
Article in BMC cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 40 citations in OpenAlex.
- Liver endothelial cells in NAFLD and transition to NASH and HCC.Cellular and molecular life sciences : CMLS · 2023Review
- The wHole Story About Fenestrations in LSEC.Frontiers in physiology · 2021Review
- Bioactive Compounds from Marine Heterobranchs.Marine drugs · 2020Review
- Bioactive compounds from marine invertebrates as potent anticancer drugs: the possible pharmacophores modulating cell death pathways.Molecular biology reports · 2020Review
- Marine Mollusk-Derived Agents with Antiproliferative Activity as Promising Anticancer Agents to Overcome Chemotherapy Resistance.Medicinal research reviews · 2017Review
- A Sieve-Raft Hypothesis for the regulation of endothelial fenestrations.Computational and structural biotechnology journal · 2013Review
- Multi-dimensional correlative imaging of subcellular events: combining the strengths of light and electron microscopy.Biophysical reviews · 2010Review
- Glomerular endothelial cell fenestrations: an integral component of the glomerular filtration barrier.American journal of physiology. Renal physiology · 2009Review
- A selective account of effective paradigms and significant outcomes in the discovery of inspirational marine natural products.Journal of natural products · 2009Review
- Vascular endothelial growth factor attenuates hepatic sinusoidal capillarization in thioacetamide-induced cirrhotic rats.World journal of gastroenterology · 2008Article
- Reconstruction of liver organoid using a bioreactor.World journal of gastroenterology · 2006Article
- The effect of cytochalasin B - Loaded liposomes on the ultrastructure of the liver sieve.Comparative hepatology · 2004Article
- Structural and functional aspects of liver sinusoidal endothelial cell fenestrae: a review.Comparative hepatology · 2002Article
- Antiplasmodial marine natural products in the perspective of current chemotherapy and prevention of malaria: a review.Marine biotechnology (New York, N.Y.)Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLiver sinusoidal endothelial cells (LSECs) react to different anti-actin agents by increasing their number of fenestrae. A new structure related to fenestrae formation could be observed when LSECs were treated with misakinolide. In this study, we investigated the effects of two new actin-binding agents on fenestrae dynamics. High-resolution microscopy, including immunocytochemistry and a combination of fluorescence- and scanning electron microscopy was applied.
resultsHalichondramide and dihydrohalichondramide disrupt microfilaments within 10 minutes and double the number of fenestrae in 30 minutes. Dihydrohalichondramide induces fenestrae-forming centers, whereas halichondramide only revealed fenestrae-forming centers without attached rows of fenestrae with increasing diameter. Correlative microscopy showed the absence of actin filaments (F-actin) in sieve plates and fenestrae-forming centers. Comparable experiments on umbilical vein endothelial cells and bone marrow sinusoidal endothelial cells revealed cell contraction without the appearance of fenestrae or fenestrae-forming centers.
conclusion(I) A comparison of all anti-actin agents tested so far, revealed that the only activity that misakinolide and dihydrohalichondramide have in common is their barbed end capping activity; (II) this activity seems to slow down the process of fenestrae formation to such extent that it becomes possible to resolve fenestrae-forming centers; (III) fenestrae formation resulting from microfilament disruption is probably unique to LSECs.
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