ArticleRNA (New York, N.Y.)2002
Mutations in RRM4 uncouple the splicing repression and RNA-binding activities of polypyrimidine tract binding protein.
Article in RNA (New York, N.Y.), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 57 citations in OpenAlex.
- Integrative structural analysis of NF45-NF90 heterodimers reveals architectural rearrangements and oligomerization on binding dsRNA.Nucleic acids research · 2025Article
- Integrative solution structure of PTBP1-IRES complex reveals strong compaction and ordering with residual conformational flexibility.Nature communications · 2023Article
- Reviewing PTBP1 Domain Modularity in the Pre-Genomic Era: A Foundation to Guide the Next Generation of Exploring PTBP1 Structure-Function Relationships.International journal of molecular sciences · 2023Review
- Neural Isoforms of Agrin Are Generated by Reduced PTBP1-RNA Interaction Network Spanning the Neuron-Specific Splicing Regions inInternational journal of molecular sciences · 2023Article
- SRSF1 and PTBP1 AreMolecular and cellular biology · 2019Article
- Nuclear matrix protein Matrin3 regulates alternative splicing and forms overlapping regulatory networks with PTB.The EMBO journal · 2015Article
- The organization of RNA contacts by PTB for regulation of FAS splicing.Nucleic acids research · 2014Article
- New insights into functional roles of the polypyrimidine tract-binding protein.International journal of molecular sciences · 2013Review
- Combinatorial mutagenesis of MBNL1 zinc fingers elucidates distinct classes of regulatory events.Molecular and cellular biology · 2012Article
- PSD-95 is post-transcriptionally repressed during early neural development by PTBP1 and PTBP2.Nature neuroscience · 2012Article
- Alternative Splicing of a Novel Inducible Exon Diversifies the CASK Guanylate Kinase Domain.Journal of nucleic acids · 2012Article
- Regulation of the mutually exclusive exons 8a and 8 in the CaV1.2 calcium channel transcript by polypyrimidine tract-binding protein.The Journal of biological chemistry · 2011Article
- Stoichiometry of a regulatory splicing complex revealed by single-molecule analyses.The EMBO journal · 2010Article
- RNA looping by PTB: Evidence using FRET and NMR spectroscopy for a role in splicing repression.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Next-generation SELEX identifies sequence and structural determinants of splicing factor binding in human pre-mRNA sequence.RNA (New York, N.Y.) · 2009Article
- The domains of polypyrimidine tract binding protein have distinct RNA structural preferences.Biochemistry · 2009Article
- hnRNP I inhibits Notch signaling and regulates intestinal epithelial homeostasis in the zebrafish.PLoS genetics · 2009Article
- A polypyrimidine tract binding protein, pumpkin RBP50, forms the basis of a phloem-mobile ribonucleoprotein complex.The Plant cell · 2009Article
- Characterization of multimeric complexes formed by the human PTB1 protein on RNA.RNA (New York, N.Y.) · 2006Article
- The hnRNPs F and H2 bind to similar sequences to influence gene expression.The Biochemical journal · 2006Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polypyrimidine tract binding protein (PTB, or hnRNP I) contains four RNA-binding domains of the ribonucleoprotein fold type (RRMs 1, 2, 3, and 4), and mediates the negative regulation of alternative splicing through sequence-specific binding to intronic splicing repressor elements. To assess the roles of individual RRM domains in splicing repression, a neural-specific splicing extract was used to screen for loss-of-function mutations that fail to switch splicing from the neural to nonneural pathway. These results show that three RRMs are sufficient for wild-type RNA binding and splicing repression activity, provided that RRM4 is intact. Surprisingly, the deletion of RRM4, or as few as 12 RRM4 residues, effectively uncouples these functions. Such an uncoupling phenotype is unique to RRM4, and suggests a possible regulatory role for this domain either in mediating specific RNA contacts, and/or contacts with putative splicing corepressors. Evidence of a role for RRM4 in anchoring PTB binding adjacent to the branch site is shown by mobility shift and RNA footprinting assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.