Evidence map›Paper›PMID 11910016›Full record

ArticleThe Plant cell2002

Use of the transposon Ac as a gene-searching engine in the maize genome.

Matthew Cowperthwaite, Wonkeun Park, Zhennan Xu, Xianghe Yan, Steven C Maurais, Hugo K Dooner

Open access · greenAbstract read
In one paragraph

Article in The Plant cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.

0numbers the graph read from it
0cells of the map it votes in
48citing papers in PubMed
10.9field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

48 citing papers in PubMed, 98 citations in OpenAlex.

  1. Creating a gene-indexed EMS mutation library of Zheng58 for improving maize genetics research.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025
    Article
  2. Strand asymmetries across genomic processes.Computational and structural biotechnology journal · 2023
    Review
  3. Cloning of MaizeInternational journal of molecular sciences · 2022
    Article
  4. Article
  5. Maize kernel development.Molecular breeding : new strategies in plant improvement · 2021
    Review
  6. Proceedings of the National Academy of Sciences of the United States of America · 2020
    Article
  7. Article
  8. Article
  9. An Efficient System forPlant physiology · 2019
    Article
  10. MaizeProceedings of the National Academy of Sciences of the United States of America · 2017
    Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Matthew CowperthwaiteWaksman Institute, Rutgers University, Piscataway, New Jersey 08855, USA.
Wonkeun Park
Zhennan Xu
Xianghe Yan
Steven C Maurais
Hugo K Dooner
Rutgers, The State University of New Jersey · USDepartment of Plant Biology · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We show here that, although genes constitute only a small percentage of the maize genome, it is possible to identify them phenotypically as Ac receptor sites. Simple and efficient Ac transposition assays based on the well-studied endosperm markers bz and wx were used to generate a collection of >1300 independent Ac transposants. The majority of transposed Ac elements are linked to either the bz or the wx donor loci on chromosome 9. A few of the insertions produce obvious visible phenotypes, but most of them do not, suggesting that these populations will be more useful for reverse genetics than for forward transposon mutagenesis. An inverse polymerase chain reaction method was adapted for the isolation of DNA adjacent to the transposed Ac elements (tac sites). Most Ac insertions were into unique DNA. By sequencing tac sites and comparing the sequences to existing databases, insertions were identified in a number of putative maize genes. The expression of most of these genes was confirmed by RNA gel blot analysis. We report here the isolation and characterization of the first 46 tac sites from the two insertion libraries.

Indexed as

DNA Transposable ElementsGenome, PlantComputational BiologyDNA, PlantGene ExpressionGenes, PlantGenomic LibraryMolecular Sequence DataMutagenesis, InsertionalSequence Analysis, DNAZea maysDNA, PlantDNA Transposable Elements

Identifiers

PMID11910016
PMCPMC150591
OpenAlexW2135270496

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.