ArticleThe Plant cell2002
In vivo analysis of the role of atTic20 in protein import into chloroplasts.
Article in The Plant cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Identification, cloning and expression analysis of chloroplast TOC-TIC complex components in a CBMC plant biology · 2026Article
- Article
- Tic12, a 12-kDa essential component of the translocon at the inner envelope membrane of chloroplasts in Arabidopsis.The Plant cell · 2022Article
- Comparative Plant Transcriptome Profiling of Arabidopsis thaliana Col-0 and Camelina sativa var.Microbiology spectrum · 2022Article
- Mutations in the chloroplast inner envelope protein TIC100 impair and repair chloroplast protein import and impact retrograde signaling.The Plant cell · 2022Article
- Genetic Mapping of theFrontiers in plant science · 2022Article
- Coexpressed subunits of dual genetic origin define a conserved supercomplex mediating essential protein import into chloroplasts.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Seascape Genomics of the Sugar KelpGenes · 2020Article
- Origins, function, and regulation of the TOC-TIC general protein import machinery of plastids.Journal of experimental botany · 2020Article
- Transit Peptides From Photosynthesis-Related Proteins Mediate Import of a Marker Protein Into Different Plastid Types and Within Different Species.Frontiers in plant science · 2020Article
- Sensitivity and Responses of Chloroplasts to Heat Stress in Plants.Frontiers in plant science · 2020Review
- Verification of theInternational journal of molecular sciences · 2019Article
- A Protochlorophyllide (Pchlide)Frontiers in plant science · 2019Article
- En route into chloroplasts: preproteins' way home.Photosynthesis research · 2018Review
- Genetics and Physiology of the Nuclearly Inherited Yellow Foliar Mutants in Soybean.Frontiers in plant science · 2018Review
- Suppressors of the Chloroplast Protein Import MutantThe Plant cell · 2017Article
- Import of Soluble Proteins into Chloroplasts and Potential Regulatory Mechanisms.Frontiers in plant science · 2017Review
- Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants.Journal of visualized experiments : JoVE · 2016Article
- Article
- New insights into the mechanism of chloroplast protein import and its integration with protein quality control, organelle biogenesis and development.Journal of molecular biology · 2015Review
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The import of nucleus-encoded preproteins into plastids requires the coordinated activities of membrane protein complexes that facilitate the translocation of polypeptides across the envelope double membrane. Tic20 was identified previously as a component of the import machinery of the inner envelope membrane by covalent cross-linking studies with trapped preprotein import intermediates. To investigate the role of Tic20 in preprotein import, we altered the expression of the Arabidopsis Tic20 ortholog (atTic20) by antisense expression. Several antisense lines exhibited pronounced chloroplast defects exemplified by pale leaves, reduced accumulation of plastid proteins, and significant growth defects. The severity of the phenotypes correlated directly with the reduction in levels of atTic20 expression. In vitro import studies with plastids isolated from control and antisense plants indicated that the antisense plastids are defective specifically in protein translocation across the inner envelope membrane. These data suggest that Tic20 functions as a component of the protein-conducting channel at the inner envelope membrane.
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