ArticleMolecular and cellular biology2002
Kap121p-mediated nuclear import is required for mating and cellular differentiation in yeast.
Article in Molecular and cellular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 46 citations in OpenAlex.
- The molecular mechanism for nuclear transport and its application.Anatomy & cell biology · 2017Review
- The ß-importin KAP8 (Pse1/Kap121) is required for nuclear import of the cellulase transcriptional regulator XYR1, asexual sporulation and stress resistance in Trichoderma reesei.Molecular microbiology · 2015Article
- Nucleocytoplasmic transport: a role for nonspecific competition in karyopherin-nucleoporin interactions.Molecular & cellular proteomics : MCP · 2012Article
- The yeast nuclear pore complex and transport through it.Genetics · 2012Article
- Sumo-dependent substrate targeting of the SUMO protease Ulp1.BMC biology · 2011Article
- Nuclear import by karyopherin-βs: recognition and inhibition.Biochimica et biophysica acta · 2011Review
- Interaction with the histone chaperone Vps75 promotes nuclear localization and HAT activity of Rtt109 in vivo.Traffic (Copenhagen, Denmark) · 2011Article
- Ribosome synthesis-unrelated functions of the preribosomal factor Rrp12 in cell cycle progression and the DNA damage response.Molecular and cellular biology · 2011Article
- A novel mechanism for SUMO system control: regulated Ulp1 nucleolar sequestration.Molecular and cellular biology · 2010Article
- Dynamic changes in the subcellular distribution of Gpd1p in response to cell stress.The Journal of biological chemistry · 2010Article
- The nuclear export factor Xpo1p targets Mad1p to kinetochores in yeast.The Journal of cell biology · 2009Article
- Article
- Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processes.Molecular biology of the cell · 2007Article
- The role of karyopherins in the regulated sumoylation of septins.The Journal of cell biology · 2007Article
- Simple kinetic relationships and nonspecific competition govern nuclear import rates in vivo.The Journal of cell biology · 2006Article
- Interactions between Mad1p and the nuclear transport machinery in the yeast Saccharomyces cerevisiae.Molecular biology of the cell · 2005Article
- Characterization of karyopherin cargoes reveals unique mechanisms of Kap121p-mediated nuclear import.Molecular and cellular biology · 2004Article
- Intersection of the Kap123p-mediated nuclear import and ribosome export pathways.Molecular and cellular biology · 2003Article
- Karyopherins in nuclear pore biogenesis: a role for Kap121p in the assembly of Nup53p into nuclear pore complexes.The Journal of cell biology · 2002Article
- Transcriptome profiling to identify genes involved in peroxisome assembly and function.The Journal of cell biology · 2002Article
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To further our understanding of how the nucleocytoplasmic transport machinery interfaces with its cargoes and how this affects cellular physiology, we investigated the molecular mechanisms of phenotypes associated with mutations in karyopherin Kap121p. Two previously unreported phenotypes of kap121 cells were observed: defects in mating and in the transition from the normal yeast form to the pseudohyphal, invasive form. In parallel, we searched for Kap121p cargoes by using Kap121p as a probe in overlay assays of yeast nuclear proteins. One of the major interacting proteins identified by this procedure was Ste12p, a transcription factor central to both the mating response and the pseudohyphal transition. We therefore investigated whether defects in these differentiation processes were due to an inability to import Ste12p. Both immunopurification and in vitro binding studies demonstrated that Ste12p interacted specifically with Kap121p in a Ran-GTP-sensitive manner and that Ste12p was mislocalized to the cytoplasm by inactivation of Kap121p in a temperature-sensitive mutant. The Kap121p-specific nuclear localization signal (NLS) of Ste12p was determined to reside within a C-terminal region of Ste12p. Furthermore, by overexpression of STE12 or expression of a STE12-cNLS fusion in kap121 cells, the invasive-growth defect and the mating defect were both suppressed. Together these data demonstrate that Ste12p is imported into nuclei by Kap121p and that mating and differentiation defects associated with kap121 mutants are primarily attributable to the mislocalization of Ste12p.
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