ArticleMolecular biology of the cell2002
Two related subpellicular cytoskeleton-associated proteins in Trypanosoma brucei stabilize microtubules.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 46 citations in OpenAlex.
- Cell Death and Unprecedent Cytoskeletal Changes inChemical & biomedical imaging · 2026Article
- Temperature dependence of the motility of the parasitic microswimmer Trypanosoma brucei.Biophysical journal · 2026Article
- Kharon Is Crucial forPathogens (Basel, Switzerland) · 2025Article
- Morphogenesis Dynamics in Leishmania Differentiation.Pathogens (Basel, Switzerland) · 2022Article
- Novel Cytoskeleton-Associated Proteins inMicroorganisms · 2021Article
- The Trypanosoma brucei subpellicular microtubule array is organized into functionally discrete subdomains defined by microtubule associated proteins.PLoS pathogens · 2021Article
- More than Microtubules: The Structure and Function of the Subpellicular Array in Trypanosomatids.Trends in parasitology · 2019Review
- Identification of TOEFAZ1-interacting proteins reveals key regulators of Trypanosoma brucei cytokinesis.Molecular microbiology · 2018Article
- Identification of paralogous life-cycle stage specific cytoskeletal proteins in the parasite Trypanosoma brucei.PloS one · 2014Article
- More than meets the eye: understanding Trypanosoma brucei morphology in the tsetse.Frontiers in cellular and infection microbiology · 2013Review
- The Trypanosoma brucei AIR9-like protein is cytoskeleton-associated and is required for nucleus positioning and accurate cleavage furrow placement.Molecular microbiology · 2012Article
- A kinetoplastid-specific kinesin is required for cytokinesis and for maintenance of cell morphology in Trypanosoma brucei.Molecular microbiology · 2012Article
- SPM1 stabilizes subpellicular microtubules in Toxoplasma gondii.Eukaryotic cell · 2012Article
- Article
- Cytokinesis in bloodstream stage Trypanosoma brucei requires a family of katanins and spastin.PloS one · 2012Article
- Replication of the ERES:Golgi junction in bloodstream-form African trypanosomes.Molecular microbiology · 2011Article
- Morphological events during the cell cycle of Leishmania major.Eukaryotic cell · 2011Article
- Three-dimensional cellular architecture of the flagellar pocket and associated cytoskeleton in trypanosomes revealed by electron microscope tomography.Journal of cell science · 2009Article
- The RACK1 homologue from Trypanosoma brucei is required for the onset and progression of cytokinesis.The Journal of biological chemistry · 2006Article
- The developmental cell biology of Trypanosoma brucei.Journal of cell science · 2005Review
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
The subpellicular microtubules of the trypanosome cytoskeleton are cross-linked to each other and the plasma membrane, creating a cage-like structure. We have isolated, from Trypanosoma brucei, two related low-molecular-weight cytoskeleton-associated proteins (15- and 17-kDa), called CAP15 and CAP17, which are differentially expressed during the life cycle. Immunolabeling shows a corset-like colocalization of both CAPs and tubulin. Western blot and electron microscope analyses show CAP15 and CAP17 labeling on detergent-extracted cytoskeletons. However, the localization of both proteins is restricted to the anterior, microtubule minus, and less dynamic half of the corset. CAP15 and CAP17 share properties of microtubule-associated proteins when expressed in heterologous cells (Chinese hamster ovary and HeLa), colocalization with their microtubules, induction of microtubule bundle formation, cold resistance, and insensitivity to nocodazole. When overexpressed in T. brucei, both CAP15 and CAP17 cover the whole subpellicular corset and induce morphological disorders, cell cycle-based abnormalities, and subsequent asymmetric cytokinesis.
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