ArticleMolecular biology of the cell2002
Dynein supports motility of endoplasmic reticulum in the fungus Ustilago maydis.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 106 citations in OpenAlex.
- Review
- CDR2 is a dynein adaptor recruited by kinectin to regulate ER sheet organization.The Journal of cell biology · 2025Article
- Organelle activity organized by the endoplasmic reticulum-mitochondria encounter structure -ERMES- is essential forMicrobial cell (Graz, Austria) · 2025Article
- A bird's-eye view of the endoplasmic reticulum in filamentous fungi.Microbiology and molecular biology reviews : MMBR · 2024Review
- Kinesin Motors in the Filamentous Basidiomycetes in Light of theJournal of fungi (Basel, Switzerland) · 2022Review
- Article
- The GUL-1 Protein Binds Multiple RNAs Involved in Cell Wall Remodeling and Affects the MAK-1 Pathway inFrontiers in fungal biology · 2021Article
- Off the wall: The rhyme and reason ofCell surface (Amsterdam, Netherlands) · 2019Review
- A novel, dynein-independent mechanism focuses the endoplasmic reticulum around spindle poles in dividing Drosophila spermatocytes.Scientific reports · 2019Article
- Fluorescent markers of various organelles in the wheat pathogen Zymoseptoria tritici.Fungal genetics and biology : FG & B · 2017Article
- Structural reorganization of the fungal endoplasmic reticulum upon induction of mycotoxin biosynthesis.Scientific reports · 2017Article
- Peroxisomes, lipid droplets, and endoplasmic reticulum "hitchhike" on motile early endosomes.The Journal of cell biology · 2015Article
- A protein complex containing Epo1p anchors the cortical endoplasmic reticulum to the yeast bud tip.The Journal of cell biology · 2015Article
- Cellular compartmentalization of secondary metabolism.Frontiers in microbiology · 2015Review
- Hook is an adapter that coordinates kinesin-3 and dynein cargo attachment on early endosomes.The Journal of cell biology · 2014Article
- Early endosome motility spatially organizes polysome distribution.The Journal of cell biology · 2014Article
- Multiple mechanisms determine ER network morphology during the cell cycle in Xenopus egg extracts.The Journal of cell biology · 2013Article
- Neurospora crassa NKIN2, a kinesin-3 motor, transports early endosomes and is required for polarized growth.Eukaryotic cell · 2013Article
- The NADPH oxidase complexes in Botrytis cinerea: evidence for a close association with the ER and the tetraspanin Pls1.PloS one · 2013Article
- The Aspergillus nidulans peripheral ER: disorganization by ER stress and persistence during mitosis.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The endoplasmic reticulum (ER) of most vertebrate cells is spread out by kinesin-dependent transport along microtubules, whereas studies in Saccharomyces cerevisiae indicated that motility of fungal ER is an actin-based process. However, microtubules are of minor importance for organelle transport in yeast, but they are crucial for intracellular transport within numerous other fungi. Herein, we set out to elucidate the role of the tubulin cytoskeleton in ER organization and dynamics in the fungal pathogen Ustilago maydis. An ER-resident green fluorescent protein (GFP)-fusion protein localized to a peripheral network and the nuclear envelope. Tubules and patches within the network exhibited rapid dynein-driven motion along microtubules, whereas conventional kinesin did not participate in ER motility. Cortical ER organization was independent of microtubules or F-actin, but reformation of the network after experimental disruption was mediated by microtubules and dynein. In addition, a polar gradient of motile ER-GFP stained dots was detected that accumulated around the apical Golgi apparatus. Both the gradient and the Golgi apparatus were sensitive to brefeldin A or benomyl treatment, suggesting that the gradient represents microtubule-dependent vesicle trafficking between ER and Golgi. Our results demonstrate a role of cytoplasmic dynein and microtubules in motility, but not peripheral localization of the ER in U. maydis.
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