ArticlePlant molecular biology2002
Cloning and characterization of a thermal hysteresis (antifreeze) protein with DNA-binding activity from winter bittersweet nightshade, Solanum dulcamara.
Article in Plant molecular biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50 citing papers in PubMed, 138 citations in OpenAlex.
- Molecular Mechanisms Underlying Freezing Tolerance in Plants: Implications for Cryopreservation.International journal of molecular sciences · 2024Review
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- RhizosphericFrontiers in plant science · 2021Article
- WRKY22 and WRKY25 transcription factors are positive regulators of defense responses in Nicotiana benthamiana.Plant molecular biology · 2021Article
- Transcriptome Analysis Reveals the Important Role ofFrontiers in plant science · 2021Article
- Ice-binding proteins confer freezing tolerance in transgenic Arabidopsis thaliana.Plant biotechnology journal · 2017Article
- Ice-Binding Proteins in Plants.Frontiers in plant science · 2017Review
- Novel Genomic and Evolutionary Insight of WRKY Transcription Factors in Plant Lineage.Scientific reports · 2016Article
- Drought-responsive WRKY transcription factor genes TaWRKY1 and TaWRKY33 from wheat confer drought and/or heat resistance in Arabidopsis.BMC plant biology · 2016Article
- A cold-tolerant evergreen interspecific hybrid of Ocimum kilimandscharicum and Ocimum basilicum: analyzing trichomes and molecular variations.Protoplasma · 2016Article
- Knockdown of Ice-Binding Proteins in Brachypodium distachyon Demonstrates Their Role in Freeze Protection.PloS one · 2016Article
- Article
- Identification and expression analysis of WRKY family genes under biotic and abiotic stresses in Brassica rapa.Molecular genetics and genomics : MGG · 2015Article
- WRKY proteins: signaling and regulation of expression during abiotic stress responses.TheScientificWorldJournal · 2015Review
- Expression and Functional Analysis of WRKY Transcription Factors in Chinese Wild Hazel, Corylus heterophylla Fisch.PloS one · 2015Article
- Global analysis of WRKY transcription factor superfamily in Setaria identifies potential candidates involved in abiotic stress signaling.Frontiers in plant science · 2015Article
- Antifreeze proteins enable plants to survive in freezing conditions.Journal of biosciences · 2014Review
- Genome-wide analysis of the WRKY gene family in cotton.Molecular genetics and genomics : MGG · 2014Article
- Antivirulence properties of an antifreeze protein.Cell reports · 2014Article
- Genome-wide identification of WRKY family genes and their response to cold stress in Vitis vinifera.BMC plant biology · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gene for a thermal hysteresis (antifreeze) protein (sthp-64) from the bittersweet nightshade, Solanum dulcamara, was cloned and characterized. An expression cDNA library prepared from November S. dulcamara was screened using a polyclonal antibody generated against a previously purified 67 kDa thermal hysteresis protein, and positive clones were identified and sequenced. The full-length thermal hysteresis protein gene was cloned into an Escherichia coli expression vector and expressed as a fusion protein. The putative thermal hysteresis protein (STHP-64) contains two conserved regions 56 and 57 amino acids in length which have the C-X4-C-X22-23-H-X1-H zinc finger motif which is present in WRKY proteins, a family of transcription factors which play a role in regulating expression of pathogenesis-related proteins in plants. Additional features of transcription factors, such as an acidic domain between the two zinc-fingers and a glutamine-rich region upstream of the first zinc-finger are also present in STHP-64. A DNA binding assay showed that the expressed STHP-64 fusion protein has specific DNA-binding ability. A unique feature of STHP-64 is that the C-terminus contains 10 consecutive 13-mer repeats. Such repeats are a common feature of animal antifreeze proteins. The expressed STHP-64 fusion protein had low levels of thermal hysteresis activity, but this activity was considerably increased by addition of citrate, which is known as an enhancer of certain insect antifreeze proteins. Northern blots demonstrated that the STHP-64 transcript was not present in leaves until November and December, suggesting that cold acclimation induces STHP-64 production.
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