ArticleProceedings of the National Academy of Sciences of the United States of America2002
Immortalization of a primate bipotent epithelial liver stem cell.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 69 citations in OpenAlex.
- Transduction of Human Fetal Liver Hematopoietic CD34+ Stem and Progenitor Cells into a Cell Line by Enhancing Telomerase Activity.International journal of hematology-oncology and stem cell research · 2024Article
- Redox-Dependent Modulation of Human Liver Progenitor Cell Line Fate.International journal of molecular sciences · 2023Article
- Successful Derivation of Hepatoblasts, Cholangiocytes and Hepatocytes from Simian Induced Pluripotent Stem Cells.International journal of molecular sciences · 2022Article
- Inhibition of nuclear factor (erythroid-derived 2)-like 2 promotes hepatic progenitor cell activation and differentiation.NPJ Regenerative medicine · 2021Article
- Utility of Common Marmoset (Genes · 2020Review
- Immortalized common marmoset (Cell discovery · 2018Article
- Integration-deficient lentivectors: an effective strategy to purify and differentiate human embryonic stem cell-derived hepatic progenitors.BMC biology · 2013Article
- Human pluripotent stem cells for modelling human liver diseases and cell therapy.Current gene therapy · 2013Review
- Spatial relationship between expression of cytokeratin-19 and that of connexin-43 in human fetal kidney.Anatomy & cell biology · 2013Article
- Ontogenesis of hepatic and pancreatic stem cells.Stem cell reviews and reports · 2012Review
- Side Population Cells From an Immortalized Human Liver Epithelial Cell Line Exhibit Hepatic Stem-Like Cell Properties.Cell medicine · 2012Article
- Progress in stem cell-derived technologies for hepatocellular carcinoma.Stem cells and cloning : advances and applications · 2010Review
- Translational control plays a prominent role in the hepatocytic differentiation of HepaRG liver progenitor cells.Genome biology · 2008Article
- Stem cells in the adult pancreas and liver.The Biochemical journal · 2007Review
- Which are the right cells to be used in a bioartificial liver?Metabolic brain disease · 2005Review
- Hepatocyte progenitors in man and in rodents--multiple pathways, multiple candidates.International journal of experimental pathology · 2005Review
- Long-term controlled immortalization of a primate hepatic progenitor cell line after Simian virus 40 T-Antigen gene transfer.Oncogene · 2005Article
- Does the reservoir for self-renewal stem from the ends?Oncogene · 2004Review
- Bipotential mouse embryonic liver stem cell lines contribute to liver regeneration and differentiate as bile ducts and hepatocytes.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- A novel immortalization vector for the establishment of penaeid shrimp cell lines.In vitro cellular & developmental biology. AnimalArticle
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Liver regeneration after partial hepatectomy results primarily from the simple division of mature hepatocytes. However, during embryonic and fetal development or in circumstances under which postnatal hepatocytes are injured, organ regeneration is believed to occur from a compartment of epithelial liver stem or progenitor cells with biliary and hepatocytic bipotentiality. The ability to identify, isolate, and transplant epithelial liver stem cells from fetal liver would greatly facilitate the treatment of hepatic diseases currently requiring orthotopic liver transplantation. Here we report the identification and immortalization by retrovirus-mediated transfer of the simian virus 40 large T antigen gene of primate fetal epithelial liver cells with a dual hepatocytic biliary phenotype. These cells grow indefinitely in vitro and express the liver epithelial cell markers cytokeratins 8/18, the hepatocyte-specific markers albumin and alpha-fetoprotein, and the biliary-specific markers cytokeratins 7 and 19. Bipotentiality of gene expression was confirmed by clonal analysis initiated from single cells. Endogenous telomerase also is expressed constitutively. After orthotopic transplantation via the portal vein, approximately 50% of the injected cells integrated into the liver parenchyma of athymic mice without tumorigenicity. Three weeks after transplantation, cells having seeded in the liver parenchyma expressed both albumin and alpha-fetoprotein but had lost expression of cytokeratin 19. These results provide strong evidence for the existence of a bipotent epithelial liver stem cell in nonhuman primates. This unlimited source of donor cells also should enable the establishment of a model of allogenic liver cell transplantation in a large animal closely related to humans and shed light on important questions related to liver organogenesis and differentiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.