ArticleProceedings of the National Academy of Sciences of the United States of America2002
Mammalian germ-line transgenesis by transposition.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
76 citing papers in PubMed, 197 citations in OpenAlex.
- Transgenesis and Genome Engineering: A Historical Review.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Historical DNA Manipulation Overview.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Contemporary Transposon Tools: A Review and Guide through Mechanisms and Applications ofInternational journal of molecular sciences · 2021Review
- Potential of transposon-mediated cellular reprogramming towards cell-based therapies.World journal of stem cells · 2020Review
- The molecular biology of medulloblastoma metastasis.Brain pathology (Zurich, Switzerland) · 2020Review
- A single amino acid switch converts the Sleeping Beauty transposase into an efficient unidirectional excisionase with utility in stem cell reprogramming.Nucleic acids research · 2020Article
- RNAi combining Sleeping Beauty transposon system inhibits ex vivo expression of foot-and-mouth disease virus VP1 in transgenic sheep cells.Scientific reports · 2017Article
- Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.Scientific reports · 2017Article
- Nanotechnologies in delivery of mRNA therapeutics using nonviral vector-based delivery systems.Gene therapy · 2017Review
- Assessing Tn5 and Sleeping Beauty for transpositional transgenesis by cytoplasmic injection into bovine and ovine zygotes.PloS one · 2017Article
- Sleeping Beauty transposon system for genetic etiological research and gene therapy of cancers.Cancer biology & therapy · 2015Review
- Vector modifications to eliminate transposase expression following piggyBac-mediated transgenesis.Scientific reports · 2014Article
- mRNA-based therapeutics--developing a new class of drugs.Nature reviews. Drug discovery · 2014Review
- Gene transfer and mutagenesis mediated by Sleeping Beauty transposon in Nile tilapia (Oreochromis niloticus).Transgenic research · 2013Article
- Transposon-mediated transgenesis, transgenic rescue, and tissue-specific gene expression in rodents and rabbits.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2013Article
- Insertional mutagenesis by a hybrid piggyBac and sleeping beauty transposon in the rat.Genetics · 2012Article
- Hyperactive self-inactivating piggyBac for transposase-enhanced pronuclear microinjection transgenesis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Completion of the swine genome will simplify the production of swine as a large animal biomedical model.BMC medical genomics · 2012Article
- Hyperactive piggyBac gene transfer in human cells and in vivo.Human gene therapy · 2012Article
- Precision editing of large animal genomes.Advances in genetics · 2012Review
16 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transposons have been used in invertebrates for transgenesis and insertional mutagens in genetic screens. We tested a functional transposon called Sleeping Beauty in the one-cell mouse embryo. In this report, we describe experiments in which transposon vectors were injected into one-cell mouse embryos with mRNA expressing the SB10 transposase enzyme. Molecular evidence of transposition was obtained by cloning of insertion sites from multiple transgenic mice produced by SB10 mRNA/transposon coinjection. We also demonstrate germ-line transmission and expression from transposed elements. This technique has promise as a germ-line transgenesis method in other vertebrate species and for insertional mutagenesis in the mouse.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.