ArticleThe Biochemical journal2002
Structure determination of the exopolysaccharide produced by Lactobacillus rhamnosus strains RW-9595M and R.
Article in The Biochemical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 83 citations in OpenAlex.
- Unveiling a novel exopolysaccharide produced by Pseudomonas alcaligenes Med1 isolated from a Chilean hot spring as biotechnological additive.Scientific reports · 2024Article
- Encapsulation and stabilization of lactoferrin in polyelectrolyte ternary complexes.Food hydrocolloids · 2023Article
- Statistical Optimization of Novel Medium to Maximize the Yield of Exopolysaccharide FromFrontiers in nutrition · 2022Article
- Lactic Acid Bacteria Exopolysaccharides Producers: A Sustainable Tool for Functional Foods.Foods (Basel, Switzerland) · 2021Review
- Characterization of lactic acid bacteria derived exopolysaccharides for use as a defined neuroprotective agent against amyloid betaScientific reports · 2020Article
- Fermentation of Wheat Bran and Whey Permeate by Mono-Cultures ofFrontiers in bioengineering and biotechnology · 2020Article
- A Novel Rhamnose-Rich Hetero-exopolysaccharide Isolated from Lactobacillus paracasei DG Activates THP-1 Human Monocytic Cells.Applied and environmental microbiology · 2017Article
- Characterization of the LP28 strain-specific exopolysaccharide biosynthetic gene cluster found in the whole circular genome ofBiochemistry and biophysics reports · 2016Article
- Distinct immunomodulation of bone marrow-derived dendritic cell responses to Lactobacillus plantarum WCFS1 by two different polysaccharides isolated from Lactobacillus rhamnosus LOCK 0900.Applied and environmental microbiology · 2014Article
- Immune Modulation Capability of Exopolysaccharides Synthesised by Lactic Acid Bacteria and Bifidobacteria.Probiotics and antimicrobial proteins · 2012Article
- Identification of a Gene Cluster for the Biosynthesis of a Long, Galactose-Rich Exopolysaccharide in Lactobacillus rhamnosus GG and Functional Analysis of the Priming Glycosyltransferase.Applied and environmental microbiology · 2009Article
- Suppressive effect on activation of macrophages by Lactobacillus casei strain Shirota genes determining the synthesis of cell wall-associated polysaccharides.Applied and environmental microbiology · 2008Article
- Biodiversity of exopolysaccharides produced by Streptococcus thermophilus strains is reflected in their production and their molecular and functional characteristics.Applied and environmental microbiology · 2004Article
- Consensus-degenerate hybrid oligonucleotide primers for amplification of priming glycosyltransferase genes of the exopolysaccharide locus in strains of the Lactobacillus casei group.Applied and environmental microbiology · 2003Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Exopolysaccharides (EPSs) were isolated and purified from Lactobacillus rhamnosus strains RW-9595M, which has been shown to possess cytokine-stimulating activity, and R grown under various fermentation conditions (carbon source, incubation temperature and duration). Identical (1)H NMR spectra were obtained in all cases. Molecular masses were determined by gel permeation chromatography. The primary structure was elucidated using chemical and spectroscopic techniques. Organic acid, monosaccharide and absolute configuration analyses gave the following composition: pyruvate, 1; D-glucose, 2; D-galactose, 1; and l-rhamnose, 4. Methylation analysis indicated the presence of three residues of 3-linked rhamnose, and one residue each of 2,3-linked rhamnose, 2-linked glucose, 3-linked glucose and 4,6-linked galactose. The EPS was submitted to periodate oxidation followed by borohydride reduction. Monosaccharide analysis of the resulting polysaccharide gave the new composition: rhamnose, 4; and glucose, 1. Methylation analysis confirmed the loss of the 2-linked glucose and 4,6-linked galactose residues. On the basis of one- and two-dimensional (1)H and (13)C NMR data, the structure of the native EPS was consistent with the following heptasaccharide repeating unit: [3Rha alpha-3Glc beta-3[Gal4,6(R)Py alpha-2]Rha alpha-3Rha alpha-3Rha alpha-2Glc alpha-](n) where Rha corresponds to rhamnose (6-deoxymannose) and Py corresponds to pyruvate acetal. Complete (1)H and (13)C assignments are reported for the native and the corresponding pyruvate-hydrolysed polysaccharide. Electrospray MS and MS/MS data are given for the oligosaccharide produced by Smith degradation.
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