ArticleStem cells (Dayton, Ohio)2002
Analysis of different promoter systems for efficient transgene expression in mouse embryonic stem cell lines.
Article in Stem cells (Dayton, Ohio), 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 151 citations in OpenAlex.
- The Olfr151 Odorant Receptor Gene is Resistant to Activation in Embryonic Stem Cells.bioRxiv : the preprint server for biology · 2026Article
- Human stem cell-specific epigenetic signatures control transgene expression.Biochimica et biophysica acta. Gene regulatory mechanisms · 2024Article
- Suitable Promoter for DNA Vaccination Using a pDNA Ternary Complex.Pharmaceutics · 2024Article
- Increased prime edit rates in KCNQ2 and SCN1A via single nicking all-in-one plasmids.BMC biology · 2023Article
- Efficient and rapid fluorescent protein knock-in with universal donors in mouse embryonic stem cells.Development (Cambridge, England) · 2023Article
- A review of the tortuous path of nonviral gene delivery and recent progress.International journal of biological macromolecules · 2021Review
- DNA damage in embryonic neural stem cell determines FTLDs' fate via early-stage neuronal necrosis.Life science alliance · 2021Article
- Scaffold-Mediated Gene Delivery for Osteochondral Repair.Pharmaceutics · 2020Review
- An Optimized Protocol for Human Direct Cardiac Reprogramming.STAR protocols · 2020Article
- Non-Viral in Vitro Gene Delivery: It is Now Time to Set the Bar!Pharmaceutics · 2020Review
- A transient reporter for editing enrichment (TREE) in human cells.Nucleic acids research · 2019Article
- AAV9 Edits Muscle Stem Cells in Normal and Dystrophic Adult Mice.Molecular therapy : the journal of the American Society of Gene Therapy · 2019Article
- Article
- Using a Commonly Down-Regulated Cytomegalovirus (CMV) Promoter for High-Level Expression of Ectopic Gene in a Human B Lymphoma Cell Line.Medical science monitor : international medical journal of experimental and clinical research · 2017Article
- Blastocyst Formation Rate and Transgene Expression are Associated with Gene Insertion into Safe and Non-Safe Harbors in the Cattle Genome.Scientific reports · 2017Article
- Differential control of retrovirus silencing in embryonic cells by proteasomal regulation of the ZFP809 retroviral repressor.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Review
- Bidirectional Promoter Engineering for Single Cell MicroRNA Sensors in Embryonic Stem Cells.PloS one · 2016Article
- Defining inhibitory neurone function in respiratory circuits: opportunities with optogenetics?The Journal of physiology · 2015Review
- Excision of translesion synthesis errors orchestrates responses to helix-distorting DNA lesions.The Journal of cell biology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Mouse embryonic stem (ES) cells are derived from the inner cell mass of the preimplantation embryo and have the developmental capacity to generate all cell types of the body. Combined with efficient genetic manipulation and in vitro differentiation procedures, ES cells are a useful system for the molecular analysis of developmental pathways. We analyzed and compared the transcriptional activities of a cellular polypeptide chain elongation factor 1 alpha (EF), a cellular-virus hybrid (cytomegalo-virus [CMV] immediate early enhancer fused to chicken beta-actin [CBA]), and a viral CMV promoter system in two ES cell lines. When transiently transfected, the EF and CBA promoters robustly drove reporter gene expression, while the CMV promoter was inactive. We also demonstrated that the EF and CBA promoters effectively drove gene expression in different stages of cell development: naïve ES cells, embryoid bodies (EBs), and neuronal precursor cells. In contrast, the CMV promoter did not have transcriptional activity in either ES cells or EB but had significant activity once ES cells differentiated into neuronal precursors. Our data show that individual promoters have different abilities to express reporter gene expression in the ES and other cell types tested.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.