ArticleGene therapy2002
Transduction of human neural progenitor cells using recombinant adeno-associated viral vectors.
Article in Gene therapy, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 51 citations in OpenAlex.
- Lentiviral vector-mediated transduction of adult neural stem/progenitor cells isolated from the temporal tissues of epileptic patients.Iranian journal of basic medical sciences · 2020Article
- Amelioration of Penetrating Ballistic-Like Brain Injury Induced Cognitive Deficits after Neuronal Differentiation of Transplanted Human Neural Stem Cells.Journal of neurotrauma · 2017Article
- Therapeutic potential of human olfactory bulb neural stem cells for spinal cord injury in rats.Spinal cord · 2016Article
- Using magnetic nanoparticles for gene transfer to neural stem cells: stem cell propagation method influences outcomes.Journal of functional biomaterials · 2015Article
- Ciliary neurotrophic factor receptor regulation of adult forebrain neurogenesis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Elevated Expression of Serotonin 5-HT(2A) Receptors in the Rat Ventral Tegmental Area Enhances Vulnerability to the Behavioral Effects of Cocaine.Frontiers in psychiatry · 2013Article
- Rapid transgene expression in multiple precursor cell types of adult rat subventricular zone mediated by adeno-associated type 1 vectors.Human gene therapy · 2012Article
- Human neural stem cells differentiate and promote locomotor recovery in an early chronic spinal cord injury NOD-scid mouse model.PloS one · 2010Article
- Human neural stem cell grafts in the spinal cord of SOD1 transgenic rats: differentiation and structural integration into the segmental motor circuitry.The Journal of comparative neurology · 2009Article
- Long-term transgene expression in mouse neural progenitor cells modified with phiC31 integrase.Journal of neuroscience methods · 2008Article
- Gene transfer into primary cultures of fetal neural stem cells by a recombinant adenovirus carrying the gene for green fluorescent protein.Journal of Zhejiang University. Science. B · 2008Article
- Neurodegeneration and cell replacement.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2008Review
- Adeno-associated virus type 4 (AAV4) targets ependyma and astrocytes in the subventricular zone and RMS.Gene therapy · 2005Article
- Recombinant AAV2 transduction of primitive human hematopoietic stem cells capable of serial engraftment in immune-deficient mice.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Light-activated gene transduction of recombinant adeno-associated virus in human mesenchymal stem cells.Gene therapy · 2004Article
- Adeno-associated viral transfer of opioid receptor gene to primary sensory neurons: a strategy to increase opioid antinociception.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human neural progenitor cells (hNPCs) represent an attractive source for cell therapy of neurological disorders. Genetic modification of hNPCs may allow a controlled release of therapeutic proteins, suppress immune rejection, or produce essential neurotransmitters. In search of an effective gene delivery vehicle, we evaluated the efficiency of a recombinant adeno-associated viral (rAAV) vector expressing enhanced green fluorescent protein (CAGegfp). Our study demonstrated that CAGegfp efficiently transduced both proliferating and differentiated hNPCs in vitro. EGFP expression was detected as early as 1 day after exposure to CAGegfp and was detectable for up to 4 months. Following transduction, the growth rate of hNPCs slowed down, but they were still able to differentiate into neurons and glia. Furthermore, CAGegfp-modified hNPCs survived, differentiated and expressed EGFP after transplanting into spinal cord of adult rats. Our results indicated that rAAV vectors might be a useful tool in hNPC-based cell and gene therapy for neurological disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.