ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2002
Episodic bursting activity and response to excitatory amino acids in acutely dissociated gonadotropin-releasing hormone neurons genetically targeted with green fluorescent protein.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
52 citing papers in PubMed, 131 citations in OpenAlex.
- Altered Electrophysiology and Transcriptome of GnRH Neurons in Middle-Aged Female Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Hypothalamic Kisspeptin Neurons: Integral Elements of the GnRH System.Reproductive sciences (Thousand Oaks, Calif.) · 2023Review
- Article
- The electrophysiologic properties of gonadotropin-releasing hormone neurons.Journal of neuroendocrinology · 2022Review
- Differential Roles of Hypothalamic AVPV and Arcuate Kisspeptin Neurons in Estradiol Feedback Regulation of Female Reproduction.Neuroendocrinology · 2020Review
- Modulation of Gonadotropin-Releasing Hormone Neuron Activity and Secretion in Mice by Non-peptide Neurotransmitters, Gasotransmitters, and Gliotransmitters.Frontiers in endocrinology · 2019Review
- Progress and Challenges in the Search for the Mechanisms of Pulsatile Gonadotropin-Releasing Hormone Secretion.Frontiers in endocrinology · 2017Review
- Optogenetic activation of GnRH neurons reveals minimal requirements for pulsatile luteinizing hormone secretion.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Episodic hormone secretion: a comparison of the basis of pulsatile secretion of insulin and GnRH.Endocrine · 2014Review
- Transient and sustained afterdepolarizations in accessory olfactory bulb mitral cells are mediated by distinct mechanisms that are differentially regulated by neuromodulators.Frontiers in cellular neuroscience · 2014Article
- Kisspeptin activation of TRPC4 channels in female GnRH neurons requires PIP2 depletion and cSrc kinase activation.Endocrinology · 2013Article
- Kisspeptin excitation of GnRH neurons.Advances in experimental medicine and biology · 2013Review
- Estradiol negatively modulates the pleiotropic actions of orphanin FQ/nociceptin at proopiomelanocortin synapses.Neuroendocrinology · 2013Article
- Fast scan cyclic voltammetry as a novel method for detection of real-time gonadotropin-releasing hormone release in mouse brain slices.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Two types of burst firing in gonadotrophin-releasing hormone neurones.Journal of neuroendocrinology · 2012Article
- Estradiol directly attenuates sodium currents and depolarizing afterpotentials in isolated gonadotropin-releasing hormone neurons.Brain research · 2012Article
- A simple integrative electrophysiological model of bursting GnRH neurons.Journal of computational neuroscience · 2012Article
- 17β-oestradiol regulation of gonadotrophin-releasing hormone neuronal excitability.Journal of neuroendocrinology · 2012Review
- Cross-Talk between Metabolism and Reproduction: The Role of POMC and SF1 Neurons.Frontiers in endocrinology · 2011Article
- Regulation of endogenous conductances in GnRH neurons by estrogens.Brain research · 2010Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The gonadotropin-releasing hormone (GnRH) system, considered to be the final common pathway for the control of reproduction, has been difficult to study because of a lack of distinguishing characteristics and the scattered distribution of neurons. The development of a transgenic mouse in which the GnRH promoter drives expression of enhanced green fluorescent protein (EGFP) has provided the opportunity to perform electrophysiological studies of GnRH neurons. In this study, neurons were dissociated from brain slices prepared from prepubertal female GnRH-EGFP mice. Both current- and voltage-clamp recordings were obtained from acutely dissociated GnRH neurons identified on the basis of EGFP expression. Most isolated GnRH-EGFP neurons fired spontaneous action potentials (recorded in cell-attached or whole-cell mode) that typically consisted of brief bursts (2-20 Hz) separated by 1-10 sec. At more negative resting potentials, GnRH-EGFP neurons exhibited oscillations in membrane potential, which could lead to bursting episodes lasting from seconds to minutes. These bursting episodes were often separated by minutes of inactivity. Rapid application of glutamate or NMDA increased firing activity in all neurons and usually generated small inward currents (<15 pA), although larger currents were evoked in the remaining neurons. Both AMPA and NMDA receptors mediated the glutamate-evoked inward currents. These results suggest that isolated GnRH-EGFP neurons from juvenile mice can generate episodes of repetitive burst discharges that may underlie the pulsatile secretion of GnRH, and glutamatergic inputs may contribute to the activation of endogenous bursts.
Indexed as
Identifiers
11896170PMC6758266W2120773020What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.