ArticleInfection and immunity2002
Binding component of Clostridium perfringens iota-toxin induces endocytosis in Vero cells.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 56 citations in OpenAlex.
- Review
- Pathogenicity and virulence ofVirulence · 2021Article
- Article
- Article
- Cryo-EM structures reveal translocational unfolding in the clostridial binary iota toxin complex.Nature structural & molecular biology · 2020Article
- Interaction ofToxins · 2018Article
- Mechanisms of Action and Cell Death Associated withToxins · 2018Review
- Article
- Cellular Entry of Clostridium perfringens Iota-Toxin and Clostridium botulinum C2 Toxin.Toxins · 2017Review
- Crystal structure and structure-based mutagenesis of actin-specific ADP-ribosylating toxin CPILE-a as novel enterotoxin.PloS one · 2017Article
- Roles of Asp179 and Glu270 in ADP-Ribosylation of Actin by Clostridium perfringens Iota Toxin.PloS one · 2015Article
- Clostridium and bacillus binary enterotoxins: bad for the bowels, and eukaryotic being.Toxins · 2014Review
- Tailored cyclodextrin pore blocker protects mammalian cells from clostridium difficile binary toxin CDT.Toxins · 2014Article
- Fructo-oligosaccharide attenuates the production of pro-inflammatory cytokines and the activation of JNK/Jun pathway in the lungs of D-galactose-treated Balb/cJ mice.European journal of nutrition · 2014Article
- The p38 MAPK and JNK pathways protect host cells against Clostridium perfringens beta-toxin.Infection and immunity · 2013Article
- Article
- Intracellular trafficking of Clostridium perfringens iota-toxin b.Infection and immunity · 2012Article
- Article
- Clostridium perfringens iota-toxin b induces rapid cell necrosis.Infection and immunity · 2011Article
- Membrane translocation of binary actin-ADP-ribosylating toxins from Clostridium difficile and Clostridium perfringens is facilitated by cyclophilin A and Hsp90.Infection and immunity · 2011Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium perfringens iota-toxin is a binary toxin consisting of two individual proteins, the binding component (Ib) and the enzyme component (Ia). Wild-type Ib bound to Vero cells at 4 and 37 degrees C and formed oligomers at 37 degrees C but not at 4 degrees C. The Ib-induced K(+) release from the cells was dependent on the oligomer formation of Ib in the cells, but the oligomer formation did not induce rounding activity or cytotoxicity. After incubation of the cells with recombinant Ib (rIb) at 37 degrees C, the Ib oligomer in the cell became resistant to pronase treatment with time, but the Ib monomer was sensitive to the treatment. Furthermore, treatment of Vero cells with rIb in the presence of bafilomycin, methylamine, or ethylamine resulted in accumulation of the oligomer in the cells but had no effect on K(+) release. Moreover, incubation with Ib plus Ia in the presence of these agents caused no rounding in the cells. These observations suggest that Ib binds to Vero cells, itself oligomerizing to form ion-permeable channels and that the formation of oligomer then induces endocytosis.
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Registered trials
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