ArticleInfection and immunity2002
Peripheral blood mononuclear cell activation induced by Leptospira interrogans glycolipoprotein.
Article in Infection and immunity, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 64 citations in OpenAlex.
- Leptospiral hemolysins induce proinflammatory cytokines through Toll-like receptor 2-and 4-mediated JNK and NF-κB signaling pathways.PloS one · 2012Trial
- Cellular Pathophysiology of Leptospirosis: Role of Na/K-ATPase.Microorganisms · 2023Review
- Bovine leptospirosis: effects on reproduction and an approach to research in Colombia.Tropical animal health and production · 2022Review
- Anti-Leptospira immunoglobulin profiling in mice reveals strain specific IgG and persistent IgM responses associated with virulence and renal colonization.PLoS neglected tropical diseases · 2021Article
- The monocytosis during human leptospirosis is associated with modest immune cell activation states.Medical microbiology and immunology · 2019Article
- Inferring pathogen-host interactions between Leptospira interrogans and Homo sapiens using network theory.Scientific reports · 2019Article
- Hepatic leptospiral infections in dogs without obvious renal involvement.Journal of veterinary internal medicine · 2019Article
- Severe Leptospirosis Features in the Spleen Indicate Cellular Immunosuppression Similar to That Found in Septic Shock.Frontiers in immunology · 2019Article
- Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp.PLoS neglected tropical diseases · 2018Article
- Monocyte human leukocyte antigen-DR expression-a tool to distinguish intestinal bacterial infections from inflammatory bowel disease?Shock (Augusta, Ga.) · 2013Article
- Responses of murine and human macrophages to leptospiral infection: a study using comparative array analysis.PLoS neglected tropical diseases · 2013Article
- Renal involvement in leptospirosis: the effect of glycolipoprotein on renal water absorption.PloS one · 2012Article
- Leptospira and inflammation.Mediators of inflammation · 2012Article
- Responses of human endothelial cells to pathogenic and non-pathogenic Leptospira species.PLoS neglected tropical diseases · 2010Article
- Lack of demonstrable memory T cell responses in humans who have spontaneously recovered from leptospirosis in the Peruvian Amazon.The Journal of infectious diseases · 2010Article
- Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen.Nature reviews. Microbiology · 2009Review
- Serum sialic acid and oxidative stress parameters changes in cattle with leptospirosis.Veterinary research communications · 2008Article
- Leptospirosis vaccines.Microbial cell factories · 2007Article
- High serum nitric oxide levels in patients with severe leptospirosis.Acta tropica · 2006Article
- Proinflammatory and immunomodulatory cytokine mRNA time course profiles in hamsters infected with a virulent variant of Leptospira interrogans.Infection and immunity · 2006Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leptospira interrogans glycolipoprotein (GLP) has been implicated in pathological and functional derangement seen in leptospirosis. The goal of this study was to evaluate GLP's ability to induce cellular activation, as assessed by cytokine production and expression of surface activation markers. GLP extracted from either pathogenic L. interrogans serovar Copenhageni or nonpathogenic Leptospira biflexa serovar Patoc (GLPp) was used to stimulate peripheral blood mononuclear cell cultures from healthy donors. Supernatant cytokine levels were measured by enzyme-linked immunosorbent assay. Expression of CD69 and HLA-DR on lymphocytes and monocytes, as well as lipopolysaccharide (LPS) binding, were measured by flow cytometry. At 6 h of incubation, GLP induced a significant rise in tumor necrosis factor alpha levels, which dropped progressively until 72 h of incubation. Interleukin-10 peak levels were obtained at between 24 and 48 h, with sustained levels until 72 h of incubation. The response magnitude was proportional to the GLP dose. CD69 expression on T lymphocytes and monocytes increased significantly, as did HLA-DR expression on monocytes. GLPp induced no CD69 or HLA-DR expression. GLP did not block biotinylated LPS binding to monocytes, suggesting that different pathways are used to induce cell activation. In conclusion, GLP induces cellular activation and may play a major role in the pathogenesis of leptospirosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.