ArticleCell stress & chaperones2002
Overexpression of apolipoprotein J in human fibroblasts protects against cytotoxicity and premature senescence induced by ethanol and tert-butylhydroperoxide.
Article in Cell stress & chaperones, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 84 citations in OpenAlex.
- Meta-analysis of age-related gene expression profiles identifies common signatures of aging.Bioinformatics (Oxford, England) · 2009Pooled it
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- Clusterin protects mature dendritic cells from reactive oxygen species mediated cell death.Oncoimmunology · 2024Article
- The Ins and Outs of Clusterin: Its Role in Cancer, Eye Diseases and Wound Healing.International journal of molecular sciences · 2023Review
- Long-term cardiovascular complications following sepsis: is senescence the missing link?Annals of intensive care · 2021Review
- Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss.Antioxidants (Basel, Switzerland) · 2021Article
- Missing perilymph but leaking blood-endolymph and vestibulocochlear nerve barriers in idiopathic sudden sensorineural hearing loss: A case study.Journal of otology · 2020Article
- Clusterin in Alzheimer's Disease: Mechanisms, Genetics, and Lessons From Other Pathologies.Frontiers in neuroscience · 2019Review
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- From the Cover: Sperm Molecular Biomarkers Are Sensitive Indicators of Testicular Injury following Subchronic Model Toxicant Exposure.Toxicological sciences : an official journal of the Society of Toxicology · 2016Article
- Bisphenol A disrupts transcription and decreases viability in aging vascular endothelial cells.International journal of molecular sciences · 2014Article
- Bisdemethoxycurcumin Increases Sirt1 to Antagonize t-BHP-Induced Premature Senescence in WI38 Fibroblast Cells.Evidence-based complementary and alternative medicine : eCAM · 2013Article
- Article
- Copper ability to induce premature senescence in human fibroblasts.Age (Dordrecht, Netherlands) · 2012Article
- Protective effect of clusterin on oxidative stress-induced cell death of human corneal endothelial cells.Molecular vision · 2009Article
- Derivation of a novel undifferentiated human foetal phenotype in serum-free cultures with BMP-2.Journal of cellular and molecular medicine · 2009Article
- Vanadium-induced apoptosis of HaCaT cells is mediated by c-fos and involves nuclear accumulation of clusterin.The FEBS journal · 2009Article
- Clusterin and chemoresistance.Advances in cancer research · 2009Review
- Clusterin associates with altered elastic fibers in human photoaged skin and prevents elastin from ultraviolet-induced aggregation in vitro.The American journal of pathology · 2007Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human diploid fibroblasts (HDFs) exposed to subcytotoxic stresses under H2O2, tert-butylhydroperoxide (t-BHP), and ethanol (EtOH) undergo stress-induced premature senescence (SIPS) characterized by many biomarkers of HDFs replicative senescence. Among these biomarkers are a growth arrest, an increase in the senescence-associated beta-galactosidase activity, a senescent morphology, an overexpression of p21waf-1 and the subsequent inability to phosphorylate pRb, the presence of the common 4977-bp mitochondrial deletion, and an increase in the steady-state level of several senescence-associated genes such as apolipoprotein J (apo J). Apo J has been described as a survival gene against cytotoxic stress. In order to study whether apo J would be protective against cytotoxicity SIPS and replicative senescence in human fibroblasts, a full-length complementary deoxyribonucleic acid of apo J was transfected into WI-38 HDFs and SV40-transformed WI-38 HDFs. The overexpression of apo J resulted in an increased cell survival after t-BHP and EtOH stresses at cytotoxic concentrations. In addition, when WI-38 HDFs were exposed to 5 subcytotoxic stresses with EtOH or t-BHP, in conditions that were previously shown to induce SIPS, a lower induction of 2 biomarkers of SIPS was observed in HDFs overexpressing apo J. No effect of apo J overexpression was observed on the proliferative life span of HDFs, even if apo J overexpression triggered osteonectin (SPARC) overexpression, which was shown to decrease the mitogenic potential of platelet-derived growth factor but not of other common growth-inducing conditions. Apo J senescence-related overexpression is proposed to have antiapoptotic rather than antiproliferative effects.
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