ArticleProceedings of the National Academy of Sciences of the United States of America2002
A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 141 papers.
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Who cites it
141 citing papers in PubMed, 233 citations in OpenAlex.
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- Big1 is a cell-cycle regulator linking cell size to basal body number in Tetrahymena thermophila.Current biology : CB · 2026Article
- Big1 is a cell cycle regulator linking cell size to basal body number.bioRxiv : the preprint server for biology · 2025Article
- Importin-9 and a TPR domain protein MpH drive periodic patterning of ciliary arrays in Tetrahymena.The Journal of cell biology · 2025Article
- Development of a Tetrahymena thermophila-Based Vaccine Expressing Miamiensis avidus Ciliary Proteins to Combat Scuticociliatosis.Journal of fish diseases · 2025Article
- Left-right cortical interactions drive intracellular pattern formation in the ciliate Tetrahymena.PLoS genetics · 2025Article
- Close cooperation between Semi1 and Semi2 proteins is essential for pronuclear positioning inMolecular biology of the cell · 2025Article
- The Metallothionein System inToxics · 2024Article
- VPS8D, a CORVET subunit, is required to maintain the contractile vacuole complex inbioRxiv : the preprint server for biology · 2023Article
- TheMolecular biology of the cell · 2023Article
- Snf2 Proteins Are Required to Generate Gamete Pronuclei inMicroorganisms · 2022Article
- PCD Genes-From Patients to Model Organisms and Back to Humans.International journal of molecular sciences · 2022Review
- Using a Hand-Held Gene Gun for Genetic Transformation of Tetrahymena thermophila.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Zipper head mechanism of telomere synthesis by human telomerase.Cell research · 2021Article
- A comprehensive review on microbial production of 1,2-propanediol: micro-organisms, metabolic pathways, and metabolic engineering.Biotechnology for biofuels · 2021Review
- Composition and function of the C1b/C1f region in the ciliary central apparatus.Scientific reports · 2021Article
- Ccdc113/Ccdc96 complex, a novel regulator of ciliary beating that connects radial spoke 3 to dynein g and the nexin link.PLoS genetics · 2021Article
- ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms.Molecular biology of the cell · 2020Article
81 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The Cd(2+)-inducible metallothionein (MTT1) gene was cloned from Tetrahymena thermophila. Northern blot analysis showed that MTT1 mRNA is not detectable in the absence of Cd(2+), is induced within 10 min of its addition, is expressed in proportion to its concentration, and rapidly disappears upon its withdrawal. Similarly, when the neo1 gene coding region flanked by the MTT1 gene noncoding sequences was used to disrupt the MTT1 locus, no transformants were observed in the absence of Cd(2+), and the number of transformants was proportional to increased Cd(2+) concentration. The neo3 cassette, in which the MTT1 promoter replaced the histone gene HHF1 promoter of the previously used neo2 cassette, transformed cells at much higher frequencies than neo2 and produced germ-line knockouts where neo2 had failed. Rescuing the progeny of a mating of gamma-tubulin gene, GTU1, knockout heterokaryons with a GTU1 gene inserted into the MTT1 locus yielded >75 times more transformants than rescuing with the wild-type GTU1 gene itself. When cells rescued with the MTT1-GTU1 chimeric gene were transferred to medium lacking Cd(2+), they stopped growing and had phenotypic changes indistinguishable from cells containing only disrupted GTU1 genes. Thus, it is now possible to create conditional lethal mutants and study the terminal phenotypes of null mutations for essential genes by replacing the endogenous gene with one under the control of the MTT1 promoter. The MTT1 promoter also resulted in approximately 30 times more overexpression of the IAG48[G1] surface antigen gene of the ciliate fish parasite Ichthyophthirius multifiliis than the highly expressed BTU1 promoter, accounting for approximately 1% of the total cell protein. Thus, the MTT1 promoter should enable routine over-expression of endogenous and foreign genes in Tetrahymena.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.