ArticleProceedings of the National Academy of Sciences of the United States of America2002
Element-specific localization of Drosophila retrotransposon Gag proteins occurs in both nucleus and cytoplasm.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- Genotype by sequencing identifies natural selection as a driver of intraspecific divergence in Atlantic populations of the high dispersal marine invertebrate,Ecology and evolution · 2017Article
- Drosophila: Retrotransposons Making up Telomeres.Viruses · 2017Review
- Transposable elements inMobile genetic elements · 2017Review
- Both the Exact Target Site Sequence and a Long Poly(A) Tail Are Required for Precise Insertion of the 18S Ribosomal DNA-Specific Non-Long Terminal Repeat Retrotransposon R7Ag.Molecular and cellular biology · 2016Article
- If the cap fits, wear it: an overview of telomeric structures over evolution.Cellular and molecular life sciences : CMLS · 2014Review
- Sequencing, De Novo assembly and annotation of the Colorado Potato Beetle, Leptinotarsa decemlineata, Transcriptome.PloS one · 2014Article
- Phosphorylation at serines 216 and 221 is important for Drosophila HeT-A Gag protein stability.PloS one · 2013Article
- Retrotransposons that maintain chromosome ends.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Non-long terminal repeat (non-LTR) retrotransposons: mechanisms, recent developments, and unanswered questions.Mobile DNA · 2010Article
- Gag proteins of Drosophila telomeric retrotransposons: collaborative targeting to chromosome ends.Genetics · 2010Article
- Association of nuclear localization of a long interspersed nuclear element-1 protein in breast tumors with poor prognostic outcomes.Genes & cancer · 2010Article
- Non-LTR retrotransposons encode noncanonical RRM domains in their first open reading frame.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- P bodies, stress granules, and viral life cycles.Cell host & microbe · 2008Review
- Functional endogenous LINE-1 retrotransposons are expressed and mobilized in rat chloroleukemia cells.Nucleic acids research · 2008Article
- Molecular characterization of a retrotransposon in the Rhynchosciara americana genome and its association with telomere.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2008Article
- Regulation of telomere length in Drosophila.Cytogenetic and genome research · 2008Review
- Intracellular targeting of telomeric retrotransposon Gag proteins of distantly related Drosophila species.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Effects of telomere length in Drosophila melanogaster on life span, fecundity, and fertility.Chromosoma · 2007Article
- Two retrotransposons maintain telomeres in Drosophila.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2005Review
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Many Drosophila non-long terminal repeat (LTR) retrotransposons actively transpose into internal, gene-rich regions of chromosomes but do not transpose onto chromosome ends. HeT-A and TART are remarkable exceptions; they form telomeres of Drosophila by repeated transpositions onto the ends of chromosomes and never transpose to internal regions of chromosomes. Both telomeric and nontelomeric, non-LTR elements transpose by target-primed reverse transcription, and their targets are not determined simply by DNA sequence, so it is not clear why these two kinds of elements have nonoverlapping transposition patterns. To explore roles of retrotransposon-encoded proteins in transposition, we analyzed intracellular targeting of Gag proteins from five non-LTR retrotransposons, HeT-A, TART, jockey, Doc, and I factor. All were expressed as green fluorescent protein-tagged proteins in cultured Drosophila cells. These Gag proteins have high levels of sequence similarity, but they have dramatic differences in intracellular targeting. As expected, HeT-A and TART Gags are transported efficiently to nuclei, where they show specific patterns of localization. These patterns are cell cycle-dependent, disappearing during mitosis. In contrast, only a fraction of jockey Gag moves into nuclei, whereas neither Doc nor I factor Gag is detected in the nucleus. Gags of the nontelomeric retrotransposons form characteristic clusters in the cytoplasm. These experiments demonstrate that closely related retrotransposon Gag proteins can have different intracellular localizations, presumably because they interact differently with cellular components. We suggest that these interactions reflect mechanisms by which the cell influences the level of transposition of an element.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.