ArticleJournal of bacteriology2002
Differential DNA binding of transcriptional regulator PcaU from Acinetobacter sp. strain ADP1.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Directed evolution of L-DOPA sensing and production enables efficient incorporation of an expanded genetic code into proteins.bioRxiv : the preprint server for biology · 2025Article
- Comparative Analysis of the IclR-Family of Bacterial Transcription Factors and Their DNA-Binding Motifs: Structure, Positioning, Co-Evolution, Regulon Content.Frontiers in microbiology · 2021Article
- Towards the complete proteinaceous regulome ofMicrobial genomics · 2017Article
- GenR, an IclR-type regulator, activates and represses the transcription of gen genes involved in 3-hydroxybenzoate and gentisate catabolism in Corynebacterium glutamicum.Journal of bacteriology · 2013Article
- Transcriptional regulation of the terephthalate catabolism operon in Comamonas sp. strain E6.Applied and environmental microbiology · 2010Article
- PcaO positively regulates pcaHG of the beta-ketoadipate pathway in Corynebacterium glutamicum.Journal of bacteriology · 2010Article
- 3-Hydroxyphenylpropionate and phenylpropionate are synergistic activators of the MhpR transcriptional regulator from Escherichia coli.The Journal of biological chemistry · 2009Article
- Multiple-level regulation of genes for protocatechuate degradation in Acinetobacter baylyi includes cross-regulation.Applied and environmental microbiology · 2007Article
- Deletion mutations caused by DNA strand slippage in Acinetobacter baylyi.Applied and environmental microbiology · 2006Article
- Transcriptional organization of genes for protocatechuate and quinate degradation from Acinetobacter sp. strain ADP1.Applied and environmental microbiology · 2005Article
- Bacterial transcriptional regulators for degradation pathways of aromatic compounds.Microbiology and molecular biology reviews : MMBR · 2004Review
- The homogentisate pathway: a central catabolic pathway involved in the degradation of L-phenylalanine, L-tyrosine, and 3-hydroxyphenylacetate in Pseudomonas putida.Journal of bacteriology · 2004Article
- In vivo and in vitro evidence that TtgV is the specific regulator of the TtgGHI multidrug and solvent efflux pump of Pseudomonas putida.Journal of bacteriology · 2003Article
- Transcriptional cross-regulation of the catechol and protocatechuate branches of the beta-ketoadipate pathway contributes to carbon source-dependent expression of the Acinetobacter sp. strain ADP1 pobA gene.Applied and environmental microbiology · 2003Article
- Characterization of the 4-carboxy-4-hydroxy-2-oxoadipate aldolase gene and operon structure of the protocatechuate 4,5-cleavage pathway genes in Sphingomonas paucimobilis SYK-6.Journal of bacteriology · 2003Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcriptional regulator PcaU from Acinetobacter sp. strain ADP1 governs expression of genes for protocatechuate degradation (pca genes) as a repressor or an activator depending on the levels of the inducer protocatechuate and of its own gene. PcaU is a member of the IclR protein family. Here the DNA binding properties of the purified protein are described in terms of the location of the binding sites and the affinity to these sites. Native PcaU was purified after overexpression of the pcaU gene in Escherichia coli. It is a dimer in solution. The binding site in the pcaU-pcaI intergenic region is located between the two divergent promoters covering 45 bp, which includes three perfect 10-bp repetitions. A PcaU binding site downstream of pcaU is covered by PcaU across two palindromic sequence repetitions. The affinity of PcaU for the intergenic binding sites is 50-fold higher (dissociation constant [K(d)], 0.16 nM) than the affinity for the site downstream of pcaU (K(d), 8 nM). The binding of PcaU was tested after modifications of the intergenic binding site. Removal of any external sequence repetition still allowed for specific binding of PcaU, but the affinity was significantly reduced, suggesting an important role for all three sequence repetitions in gene expression. The involvement of DNA bending in the regulatory process is suggested by the observed strong intrinsic curvature displayed by the pcaU-pcaI intergenic DNA.
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