ArticleJournal of bacteriology2002
Characterization of an ADP-ribosyltransferase toxin (AexT) from Aeromonas salmonicida subsp. salmonicida.
Article in Journal of bacteriology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 95 citations in OpenAlex.
- Aeromonas in South Asia: genomic insights into an environmental pathogen and reservoir of antimicrobial resistance.Nature communications · 2026Article
- Cytotoxicity induced by Aeromonas schubertii is orchestrated by a unique set of type III secretion system effectors.Veterinary research · 2025Article
- Antimicrobial resistance in aeromonads and new therapies targeting quorum sensing.Applied microbiology and biotechnology · 2024Review
- MQM1, a bacteriophage infecting strains of Aeromonas salmonicida subspecies salmonicida carrying Prophage 3.Virus research · 2023Article
- 14-3-3 Activated Bacterial Exotoxins AexT and ExoT Share Actin and the SH2 Domains of CRK Proteins as Targets for ADP-Ribosylation.Pathogens (Basel, Switzerland) · 2022Article
- First Record of the Rare SpeciesPathogens (Basel, Switzerland) · 2022Article
- Effect of Iron Limitation, Elevated Temperature, and Florfenicol on the Proteome and Vesiculation of the Fish PathogenMicroorganisms · 2022Article
- Pancreaticobiliary Cancers and Aeromonas Isolates Carrying Type Ⅲ Secretion System GenesFrontiers in cellular and infection microbiology · 2021Article
- Secretion Systems in Gram-Negative Bacterial Fish Pathogens.Frontiers in microbiology · 2021Review
- Comparative and Evolutionary Genomics of Isolates Provide Insight into the Pathoadaptation of Aeromonas.Genome biology and evolution · 2020Article
- Article
- Vaccine-Induced Protection Against Furunculosis Involves Pre-emptive Priming of Humoral Immunity in Arctic Charr.Frontiers in immunology · 2019Article
- 14-3-3 proteins activate Pseudomonas exotoxins-S and -T by chaperoning a hydrophobic surface.Nature communications · 2018Article
- Genome-driven evaluation and redesign of PCR tools for improving the detection of virulence-associated genes in aeromonads.PloS one · 2018Article
- Draft Genome Sequence of the Type StrainGenome announcements · 2017Article
- The mosaic architecture ofPeerJ · 2016Article
- Virulence Factors of Aeromonas hydrophila: In the Wake of Reclassification.Frontiers in microbiology · 2016Review
- Pseudomonas aeruginosa ExoT Induces Atypical Anoikis Apoptosis in Target Host Cells by Transforming Crk Adaptor Protein into a Cytotoxin.PLoS pathogens · 2015Article
- Distribution of 13 virulence genes among clinical and environmental Aeromonas spp. in Western Australia.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
- Novel bacterial ADP-ribosylating toxins: structure and function.Nature reviews. Microbiology · 2014Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An ADP-ribosylating toxin named Aeromonas salmonicida exoenzyme T (AexT) in A. salmonicida subsp. salmonicida, the etiological agent of furunculosis in fish, was characterized. Gene aexT, encoding toxin AexT, was cloned and characterized by sequence analysis. AexT shows significant sequence similarity to the ExoS and ExoT exotoxins of Pseudomonas aeruginosa and to the YopE cytotoxin of different Yersinia species. The aexT gene was detected in all of the 12 A. salmonicida subsp. salmonicida strains tested but was absent from all other Aeromonas species. Recombinant AexT produced in Escherichia coli possesses enzymatic ADP-ribosyltransferase activity. Monospecific polyclonal antibodies directed against purified recombinant AexT detected the toxin produced by A. salmonicida subsp. salmonicida and cross-reacted with ExoS and ExoT of P. aeruginosa. AexT toxin could be detected in a wild type (wt) strain of A. salmonicida subsp. salmonicida freshly isolated from a fish with furunculosis; however, its expression required contact with RTG-2 rainbow trout gonad cells. Under these conditions, the AexT protein was found to be intracellular or tightly cell associated. No AexT was found when A. salmonicida subsp. salmonicida was incubated in cell culture medium in the absence of RTG-2 cells. Upon infection with wt A. salmonicida subsp. salmonicida, the fish gonad RTG-2 cells rapidly underwent significant morphological changes. These changes were demonstrated to constitute cell rounding, which accompanied induction of production of AexT and which led to cell lysis after extended incubation. An aexT mutant which was constructed from the wt strain with an insertionally inactivated aexT gene by allelic exchange had no toxic effect on RTG-2 cells and was devoid of AexT production. Hence AexT is directly involved in the toxicity of A. salmonicida subsp. salmonicida for RTG-2 fish cells.
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